富含普罗西亚尼丁的葡萄提取物对抗格拉姆阴性毒性因子的保护作用
Roberta Maria Nicolosi1, Graziana Bonincontro1, Elena Imperia2
1Department of Environmental Biology, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy.
Antibiotics (Basel, Switzerland)
|November 24, 2023
概括
葡萄种子提取物通过减少脂多糖 (LPS) 损伤和抑制生物膜形成来保护对抗阴性细菌. 这种天然化合物有望缓解胃肠道疾病.
科学领域:
- 胃肠病学 胃肠病学
- 微生物学 微生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 通过生物膜和脂聚糖 (LPS) 的形成,阴性细菌有助于胃肠道疾病的发病.
- 葡萄种子是葡萄酒行业的副产品,具有固有的抗氧化和抗菌特性.
研究的目的:
- 调查富含素葡萄种子提取物 (prGSE) 对LPS诱导的氧化应激和上皮屏障损伤的保护作用.
- 评估prGSE在体外和体内对细菌病原体及其毒性因子的疗效.
主要方法:
- 使用高性能液体染色学 (HPLC) 和核磁共振 (NMR) 进行prGSE的分子表征.
- 在体外评估prGSE对Caco-2细胞氧化应激和表皮透性的影响,以应对LPS.
- 在体外评估prGSE对沙门氏菌Typhimurium和大肠杆菌的抗生物膜活性.
- 在 Galleria mellonella幼虫感染模型中对prGSE疗效的体内评估.
主要成果:
- prGSE显著缓解了LPS诱导的Caco-2细胞透性的改变,并减少了反应性氧物种 (ROS) 的产生.
- prGSE显示,大肠杆菌和S. Typhimurium显著抑制生物膜的形成.
- 在体内研究显示,感染后用prGSE治疗的Galleria mellonella幼虫的生存率增加.
结论:
- 葡萄丰富的葡萄提取物对格拉姆阴性细菌内毒素和生物膜具有保护作用.
- prGSE显示出作为治疗剂的潜力,可以减少由细菌病原体引起的胃肠道损伤.
关键词:
在Caco-2中使用.埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.梅罗内拉画廊 (Galleria mellonella) 是一个著名的画廊.在LPS中使用LPS.沙门氏菌 (Salmonella enterica) 的一个亚种. 输入式血清器 Typhimurium Typhimurium 是一个类型.葡萄酒 (Vitis vinifera) L. 葡萄酒 (Vitis vinifera) L. 葡萄酒 (Vitis vinifera) L. 葡萄酒 (Vitis vinifera) L. 葡萄酒 (Vitis vinifera) L. 葡萄酒 (L.抗病毒性因素 抗病毒性因素细菌生物膜是一种细菌生物膜.葡萄种子提取物葡萄种子提取物
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