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探索多药耐药Rhodococcus equi克隆2287的辅助基因组

Sonsiray Alvarez Narvaez¹, Susan Sanchez¹

¹Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA.

Antibiotics (Basel, Switzerland)

|November 24, 2023

概括

在多药耐药 (MDR) Rhodococcus equi克隆2287中发现的一种新型遗传元素IME2287可能解释了它的成功. 这种元素可能有助于这种重要的小病原体的传播和持续存在.

科学领域:

兽医微生物学兽医微生物学
细菌遗传学细菌遗传学
抗微生物耐药性抗微生物耐药性

背景情况:

过度使用小的抗微生物药物导致了多药耐药 (MDR) Rhodococcus equi. 的全球传播.
有三种MDR R. equi克隆种群 (2287,G2106,G2017),但克隆2287最常从生病的小马中分离出来.
克隆2287增加患病率和毒性的遗传基础尚不清楚.

研究的目的:

为了对R. equi分离的辅助基因组进行遗传特征.
确定有助于MDR R. equi克隆2287.7成功的新型遗传元素.
调查这些元素在克隆2287.7的维持和传播中的潜在作用.

主要方法:

对207个R. equi分离物辅助基因组的深度遗传特征.
一个新的遗传元素的识别和描述,IME2287.
分析IME2287内的基因,包括复制,切除,整合,TIR域蛋白和毒素-抗毒素系统.

主要成果:

在R. equi克隆2287.2的辅助基因组内发现IME2287,一个可谓的自我复制的整合性可调动元素 (IME).
IME2287拥有通过血清蛋白重组酶进行复制,分区和集成/切割的基因.
IME2287编码了一种TIR域蛋白,可能抑制宿主NF-kB信号传递,以及与细菌持久性和抗生素耐药性相关的毒素-抗毒素系统.

结论:

IME2287是一种新型遗传元素,可能是MDR R. equiclone 2287的成功繁殖和传播的原因.
IME2287中的基因,包括那些参与宿主免疫调节和抗生素耐受性的基因,可能有助于克隆2287的优势.
了解IME2287对于制定控制小羊中MDR R. equi感染的策略至关重要.

关键词:

IME228787 这是一个很好的例子.罗多科克斯 (Rhodococcus equi) 是一个同等的菌种.一个克隆 228787 具有多种耐药性的药物.

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