核局部光蛋白使得核行为可视化在BasidiomyceteSchizophyllum公社早期交配相互作用
Marjatta Raudaskoski1, Ciarán Butler-Hallissey2,3
1Molecular Plant Biology, Department of Life Technologies, University of Turku, FIN-20014 Turku, Finland.
Journal of fungi (Basel, Switzerland)
|November 24, 2023
概括
在 *Schizophyllum commune* 的相容交配中,核迁移受B交配类型的调节. 不同的A交配型基因对细胞周期激活和二核化至关重要,导致同步的核分裂.
科学领域:
- 菌类学 菌类学是指菌类学.
- 细胞生物学 细胞生物学
- 遗传学 是一个遗传学.
背景情况:
- 四极基底菌群 * Schizophyllum commune * 提供了一个研究真菌交配和 dikaryotization 的模型.
- 了解交配型基因 (A和B) 的遗传调节对于真菌的繁殖和发育至关重要.
研究的目的:
- 研究A型和B型交配基因在*Schizophyllum commune*中相容交配期间的核行为中的作用.
- 用光标记的基因组来可视化和分析核迁移和细胞循环激活.
主要方法:
- 旋盘共聚焦显微镜被用来观察S.commune*的活生生的交配.
- 遇到表达不同标记的2B基因素 (mCherry和EGFP) 的哈普洛伊德菌株.
- 显微镜追踪了核行为,包括在交配期间的迁移,分裂和基质子结合.
主要成果:
- 观察到菌株之间的核迁移,并发现由B交配类型调节.
- 兼容的交配 (不同的A和B基因) 导致黄色核,表明混合的组合基因子.
- 观察到A交配型转录因子的异体化和同步核分裂,导致二核化.
结论:
- 交配的B类型调节了S社区*中的核迁移.
- 不同的A交配型基因对于激活细胞循环和促进二化是必不可少的.
- 基斯顿标签提供了一个强大的工具,用于研究真菌交配期间的核动力学.
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