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来自蜜蜂病原体 (Ascosphaera apis) 的α-氨酶的表征

Jincheng Li¹, Sen Liu¹, Chenjie Yang¹

¹State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

Journal of fungi (Basel, Switzerland)

|November 24, 2023

概括

蜜蜂的粉笔繁殖真菌Ascosphaera apis产生一种强大的α-氨酶. 潜水培养条件的优化显著增强了对潜在生物技术应用的酶生产.

科学领域:

菌类学菌类学是指菌类学.
生物技术是生物技术.
酶学是一种酶学.

背景情况:

阿斯科斯费拉虫 (Ascosphaera apis) 导致蜜蜂粉病.
来自真菌的氨基酶具有重要的生物技术潜力.
了解真菌氨基酶有助于开发新的酶应用.

研究的目的:

为了优化氨基酶的生产,A. 在沉浸式文化中.
为了描述纯化的氨酶酶.
探索真菌氨基酶的生物技术应用.

主要方法:

使用Box-Behnken设计 (BBD) 进行媒体优化的响应表面方法 (RSM).
酶净化到同质性的过程.
酶性质的表征,包括热稳定性,pH活性和动力学.
生物反应器发酵用于扩大规模生产.

主要成果:

优化的介质 (46 g/L 麦芽糖,1.51 g/L CaCl,pH 6.6) 导致氨基酶活性增加了11倍.
纯化氨酶 (75 kDa) 是热稳定的,在广泛的pH范围 (7-10) 处活跃,在55°C和pH7.5.5处具有最佳活性.
这种酶是一种α-氨基酶,从粉中产生葡萄糖和麦芽糖.
生物反应器发酵实现了99.38 U/mL的酶活性.

结论:

制定了一项优化真菌酶生产的战略.
从A.A.中特征化的α-氨基酶. apis显示出生物技术应用的前景.
这项研究提供了关于真菌α-amylase的特性和生产的宝贵见解.

关键词:

阿斯科斯菲拉阿皮斯 (Ascosphaera apis) 是一种有机物.这是一种真菌病原体的病原体.大规模生产大规模生产.中等因素优化的优化.净化净化净化净化 α-氨酸酶的使用情况

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