细胞毒性,分化和生物相容性根端填充:一个全面的研究
Ignacio Jimenez-Bueno1, Rene Garcia-Contreras2, Benjamin Aranda-Herrera2
1Department of Endodontics, Faculty of Dentistry, Autonomous University State of Mexico (UAEMex), Toluca 50130, State of Mexico, Mexico.
Biomimetics (Basel, Switzerland)
|November 24, 2023
概括
用 (Portland Bi) 修改的波特兰水泥显示出良好的生物相容性,并促进了类似于口腔细胞的细胞分化,表明了内牙修复和生物仿真重矿化的潜力.
科学领域:
- 生物材料科学 生物材料科学
- 牙周内科医院 牙周内科医院 牙周内科医院
- 细胞生物学 细胞生物学
背景情况:
- 对内牙材料的生物相容性评估对于成功的根端填充至关重要.
- 了解细胞毒性,亡和自对于评估材料安全至关重要.
- 牙细胞分化是牙再生和脉修复的关键.
研究的目的:
- 评估MTA,氧化-尤根醇和实验性波特兰水泥 (波特兰Bi,波特兰Ba) 的细胞毒性和细胞反应.
- 为了研究这些材料对亡,自和细胞样细胞分化的影响.
- 确定修改后的波特兰水泥在牙周内科应用中的潜力.
主要方法:
- 初级细胞培养 (HPLF,HGF,HPC,HSC) 暴露于不同度的水泥.
- 细胞毒性通过细胞活力测试进行评估.
- 通过DNA碎片化和卡斯帕酶激活来测量亡.
- 用特定的抑制剂评估了自性.
- 诱导了类似牙细胞的分化,并分析了矿物沉积物.
主要成果:
- 所有测试的水泥都以剂量依赖的方式降低了细胞活力.
- 氧化-尤根醇没有诱导DNA碎片化,caspase-3激活或自.
- 波特兰生物显著加快了芽细胞样细胞分化 (p < 0.05).
结论:
- 用 (Portland Bi) 修改的波特兰水泥显示出细胞相容性.
- 波特兰生物活跃地促进了类似于口腔细胞细胞的细胞分化.
- 这种材料对内牙修复和生物仿真重矿化有很大的希望.
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