一种以粘土为基础的商业材料作为动物料中向性溶酶输送的载体
Marianna Guagliano1, Cinzia Cristiani1, Matteo Dell'Anno2
1Dipartimento di Chimica, Materiali e Ingegneria Chimica "Giulio Natta", Politecnico di Milano, Piazza Leonardo Da Vinci 32, 20133 Milano, Italy.
Nanomaterials (Basel, Switzerland)
|November 24, 2023
概括
这项研究提出了一种具有成本效益的方法,用于使用粘土矿物载体在动物料中保护生物活性分子,如酶 (LY). 这种方法增强了对肠道的营养递送,提高了料的效率,减少了对环境的影响.
科学领域:
- 动物营养 动物营养
- 材料科学 材料科学 材料科学
- 生物技术是生物技术.
背景情况:
- 在动物料中控制释放生物活性分子对于有效性和环境可持续性至关重要.
- 在胃肠道过境期间生物活性分子的降解仍然是一个重大挑战.
- 开发保护载体对于改善动物营养和减少环境影响至关重要.
研究的目的:
- 研究一种简单,经济高效的固体/液体吸附程序,以保护生物活性分子.
- 为优化在粘土矿物载体 (Adsorbo®) 上吸附酶 (LY) 的操作参数.
- 描述所产生的混合材料,并评估其释放特征和抗氧化能力.
主要方法:
- 固体/液体吸附过程使用酶 (LY) 作为模型生物活性分子,并使用Adsorbo® (粘土矿物质和化物) 作为载体.
- 吸附参数的优化:pH,反应时间和初始LY度.
- 使用吸附分析,电荷相互作用研究以及初步释放和抗氧化能力 (TEAC) 试验对LY-AD样本进行表征.
主要成果:
- 在优化条件下达到32mg LY/g AD的最大LY负荷 (pH7.5-8,37.5 mg LY/g AD初始度,30分钟反应时间).
- 在载体表面上LY的均分布,通过电荷进行主相互作用.
- 初步释放测试显示,在pH7下释放能力增强,LY载体复合体表现出抗氧化能力.
结论:
- 开发的吸附过程是保护动物料中的生物活性分子的可行和经济有效的方法.
- LY-AD杂交材料显示出控制释放的潜力,并改善了动物肠道中的生物可用性.
- 这种方法为提高动物营养和尽量减少对环境的影响提供了一个有希望的策略.
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