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在体外的抗炎活性和从Lobonema smithii中提取的多糖的结构特征水母

Thitikan Summat¹, Sutee Wangtueai¹, SangGuan You^2,3

¹College of Maritime Studies and Management, Chiang Mai University, Samut Sakhon 74000, Thailand.

|November 24, 2023

概括

白水母多糖,特别是JF3,通过减少氧化和关键炎症标志物,表现出强大的抗炎作用. 这表明它们作为天然治疗炎症剂的潜力.

科学领域:

海洋生物技术海洋生物技术
免疫学免疫学免疫学
自然产品化学自然产品化学

背景情况:

炎症是一种复杂的生物反应,与许多疾病有关.
海洋生物,如水母,是新生物活性化合物的潜在来源.
洛博内马·斯密希多糖 (LSPs) 尚未广泛研究其抗炎性质.

研究的目的:

从白色水母 (Lobonema smithii) 中提取和表征多糖.
为了在体外研究这些多糖的抗炎作用.
阐明其抗炎活性背后的分子机制.

主要方法:

提取水和离子交换色谱用于多糖分离.
化学表征包括单糖化合物组成和分子量测定.
评估激发脂聚糖 (LPS) 的RAW264.7巨细胞的抗炎活性,测量氧化 (NO),细胞因子表达和信号通路激活 (NF-κB,MAPK).

主要成果:

获得了四种多糖分,主要由中性糖 (葡萄糖,银糖,曼诺糖) 组成.
所有部分都减少了NO的产生;JF3显示出显著的抑制活性.
它抑制了促炎媒介 (iNOS,COX-2,IL-1β,IL-6,TNF-α),诱导了IL-10,并抑制了关键的信号通路 (NF-κB,ERK,JNK,p38).
此外,JF3还抑制了巨细胞上CD11b和CD40的表达.

结论:

洛博尼马·斯密希的多糖,特别是JF3,具有显著的抗炎性质.
JF3通过调节NF-κB和MAPK信号通路来发挥其作用.
这些发现凸显了L. smithii多糖的潜力,作为一种用于炎症疾病的新型治疗剂.

关键词:

洛博内玛·史密西 (Lobonema Smithii) 是一个非常出色的球员.这是一种抗炎药物.水母是什么意思水母是什么意思巨细胞是什么?巨细胞是什么?聚糖类是一种多糖类.

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