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在Thraustochytrium sp.中的辅酶Q. RT2316-16:介质成分的影响

Liset Flores¹, Carolina Shene¹, Juan A Asenjo²

¹Department of Chemical Engineering, Center of Food Biotechnology and Bioseparations, BIOREN, and Centre of Biotechnology and Bioengineering (CeBiB), Universidad de La Frontera, Temuco 4811230, Chile.

|November 24, 2023

概括

松虫 (Thraustochytrium sp.) 是一种有毒的植物. RT2316-16可以产生辅酶Q10 (CoQ10) 和辅酶Q9 (CoQ9). 辅酶Q10的产生受生长介质的组成和培养年龄的影响,这表明这种微生物是潜在的辅酶Q10来源.

科学领域:

生物化学生物化学
微生物学微生物学
生物技术是生物技术.

背景情况:

辅酶Q (CoQ) 对于细胞呼吸至关重要,并充当抗氧化剂.
辅酶Q10 (CoQ10) 是人类的形式,具有10个单位的多异索类链.
很少有微生物自然产生辅酶Q10,使其可持续生产成为一个关键的研究领域.

研究的目的:

为了研究 *Thraustochytrium* sp. 中的辅酶Q (CoQ) 变体的产生. RT2316-16.一个人的生活
确定生长媒介成分和培养年龄对CoQ10和CoQ9产量的影响.
为了评估 *Thraustochytrium* sp. 的潜力. RT2316-16作为可化10生产的来源.

主要方法:

种植 *Thraustochytrium* sp. 的方法 RT2316-16在不同的生长条件下.
在生物质中计量辅酶Q9 (CoQ9) 和辅酶Q10 (CoQ10) 含量.
分析特定介质成分 (例如,p-基酸,B族维生素,p-氨基酸) 对CoQ生产和细胞生长的影响.

主要成果:

* 松虫 * sp. 松虫证实RT2316-16可以产生CoQ10和CoQ9.
辅酶Q10含量随生长介质和培养年龄而有显著的变化,达到10毫克g-1.
CoQ9含量更稳定,最高为855μg-1;其功能可能与CoQ10不同.
补充p-基酸可以提高CoQ10水平.
缺少某些B族维生素和p-氨基酸会减少生长,但保留了CoQ10水平.

结论:

* 松虫 * sp. 松虫 RT2316-16是一种有前途的微生物,可用于生物技术生产CoQ10.
优化培养条件,特别是中间成分和收获时间,对于最大限度地提高CoQ10产量至关重要.
需要进一步的研究来阐明这种生物体中CoQ10合成的代谢触发因素.

关键词:

维生素B 维生素B 维生素B 松虫 (Thraustochytrium sp.) 是一种有毒的植物.达科萨赫萨埃诺酸是什么意思通过状基体的状基体这种药物是Ubiquinone.

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