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采样方法在雌激素期间对马子宫微生物组检测的作用

B A Heil^1,2, M van Heule^3,4, S K Thompson⁵

¹Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.

Veterinary sciences

|November 24, 2023

概括

在母马中检测细菌性子宫内膜炎是具有挑战性的,因为培养敏感度低. 这项研究使用16S rRNA测序揭示了马子宫微生物组,确定了低体积的洗作为检测罕见微生物的优势.

科学领域:

兽医微生物学兽医微生物学
生殖生物学生殖生物学
马类科学马类科学

背景情况:

细菌性子宫内膜炎是马不育的主要原因,影响了马匹养殖业.
目前基于培养的检测子宫微生物的方法缺乏灵敏度,导致频繁的未诊断病例.
准确的诊断对于有效治疗和减少经济损失至关重要.

研究的目的:

为了比较不同子宫内膜采样方法的有效性,以检测马匹的子宫微生物.
通过使用16S rRNA测序,在雌激发期间描述居住的马子宫微生物组.
为了确定最佳的细菌性子宫内膜炎的诊断方法.

主要方法:

十六只母马通过双防护的拭子,子宫内膜活检和低体积洗 (LVL) 进行了子宫内膜采样.
16S rRNA基因测序是在严格的质量控制和消毒后对样本进行的.
分析了Amplicon序列变异,以评估微生物组成,相对丰度和α多样性.

主要成果:

这三种采样方法都显示了在类型和属级别上类似的微生物特征.
与子宫内膜活检相比,低体积洗 (LVL) 显示出明显更高的α多样性 (Chao1).
发现LVL在检测马子宫中少量的 (罕见的) 微生物种类中优越.

结论:

16S rRNA 测序提供了在雌激发期间马子宫微生物组的全面视图.
低体积洗 (LVL) 是一种更敏感的方法,用于检测马中多样化的微生物群落,包括罕见的种类.
改进的诊断技术可以带来更好的治疗策略,以治疗马类不孕症和更明智的抗菌药物使用.

关键词:

这是子宫内膜的内分泌物.海洋的海洋海洋的海洋.微生物组是一个微生物组.生殖管道的生殖系统.采样技术采样技术这是子宫,子宫.

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