对比转录组分析揭示了通过在Actinobacillus succinogenes GXAS1377中添加NaHSO3来减少酸的分子机制
Shiyong Li1, Chaodong Song1, Hongyan Zhang1
11Guangxi Key Laboratory for Polysaccharide Materials and Modifications, Guangxi Key Laboratory of Microbial Plant Resources and Utilization, School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning, China.
Polish journal of microbiology
|November 24, 2023
概括
乙硫酸处理通过降低脂酸合成的调节和提高乙甲基醇脱酶的调节来降低酸发酵中的酸产量,揭示了关键的分子机制.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 分子生物学分子生物学
背景情况:
- 酸 (AC) 是由*Actinobacillus succinogenes*发酵酸 (SA) 的一个重要的副产品.
- 二硫酸 (NaHSO) 有效地降低了交流电的产生,但潜在的机制尚不清楚.
研究的目的:
- 为了阐明NaHSO3降低*A. succinogenes*中的AC产生的分子机制.
- 在NaHSO3治疗的反应中识别差异表达基因 (DEGs).
主要方法:
- 使用IlluminaRNA-sequencing (RNA-seq) 来比较转录的个人资料.
- 差异基因表达分析确定了210个DEG (83个上调,127个下调).
- DEGs的功能注释集中在代谢途径上.
主要成果:
- NaHSO3治疗显著降低了脂酸合成基因 (LipA,LipB) 的调节,抑制了从pyruvate中形成乙-CoA.
- 编码双功能乙甲基-酒精脱酶的基因被显著上调,可能有利于乙醇合成.
- NaHSO3可能会与乙甲发生反应,消耗乙-CoA并降低AC产量.
结论:
- 这项研究表明,NaHSO3通过破坏脂酸合成和影响乙醇脱酶活性来降低AC产量.
- 这为在SA发酵过程中*A. succinogenes*中*AC产生的调控机制提供了新的见解.
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