保存子解后的精子表观遗传修饰,生育潜力和其他质量参数在不同的扩展器使用减少的谷氨
R Masoudi1, M Hatami2, S Esmaeilkhanian1
1Animal Science Research Institute of Iran (ASRI), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran.
Theriogenology
|November 24, 2023
概括
将减少的谷氨 (GSH) 添加到精液冷保存扩展器中,可以显著改善解后的精子质量和生育能力. 补充GSH有助于维护DNA甲基化,减少氧化应激,增强生殖成功.
科学领域:
- 动物科学动物科学
- 生殖生物学 生殖生物学
- 生物化学 生物化学
背景情况:
- 精液的冷保存对于遗传传播至关重要,但可能导致生育能力下降.
- 冷保存期间的表观遗传修饰可能会对精子质量和生育能力产生负面影响.
- 氧化应激和DNA损伤是影响经解后精子生存能力的关键因素.
研究的目的:
- 调查不同冷延长剂中减少的谷氨 (GSH) 对公精子质量的影响.
- 评估解后表观遗传修饰和生育潜力的保存.
- 为了确定最佳的扩展器组成,以保持精子的生命力和功能.
主要方法:
- 的精液被稀释在湖泊和贝尔茨维尔扩展器中,有或没有减少的谷氨 (GSH).
- 精子参数包括运动性,膜完整性,线粒体活性和体完整性的精子参数在解后被评估.
- 评估了表观遗传标记 (DNA甲基化,基因素修饰),氧化应激 (ROS),DNA碎片化和生育率.
主要成果:
- 降低谷氨 (GSH) 补充剂显著改善了总运动性,渐进运动性,膜功能,线粒体活性,体完整性和活力.
- 与对照组相比,GSH治疗显示脂质过氧化,亡,DNA碎片化和ROS度降低.
- 精子DNA甲基化和生育率在用GSH补充的扩展剂中显著更高,而基因素甲基化和乙化不受影响.
结论:
- 补充减少的谷氨 (GSH) 是一种有效的策略,以提高精子的冷保存.
- GSH保留了关键的质量参数,包括DNA甲基化和生育潜力,减轻了冷损伤.
- 在扩展器中使用GSH提供了一种有前途的方法,以提高家禽养殖计划中人工授精的成功率.
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