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补充l-氨酸会扰乱重要的分子通路,并诱导小鼠丸的亡

Zi-Wei Wu¹, Li Wang¹, Qiao Mou²

¹College of Animal Science, Yangtze University, Jingzhou, 434025, Hubei, China; Center of Animal Breeding Technology Innovation of Hubei Province, China.

|November 24, 2023

概括

在雄性小鼠中补充L-氨酸会通过诱导亡和改变与自和RNA甲基化相关的基因表达来破坏丸结构. 这项研究揭示了影响男性生殖健康的潜在分子机制.

科学领域:

生殖生物学生殖生物学
分子内分泌学分子内分泌学
营养生物化学营养生物化学

背景情况:

包括瓦林在内的分支链氨基酸 (BCAA) 对动物的生长和新陈代谢至关重要.
氨酸对男性生殖功能及其分子基础的特定影响尚不清楚.

研究的目的:

研究L-瓦林补充剂对雄性小鼠生殖健康的影响.
阐明L-氨酸可能影响丸功能的分子机制.

主要方法:

雄性小鼠在饮用水中补充L-氨酸 (0.30%或0.45%) 持续3周.
对丸组织形态进行了组织学分析.
评估了与亡,自,DNA甲基化和RNA甲基化相关的基因和蛋白质表达水平.
测试转录组测序 (RNA-seq) 和RT-qPCR被用来识别差异表达的基因.

主要成果:

补充L-氨酸改变了Sertoli和生殖细胞形态,并增加了精子管内间歇空间.
高剂量的L-瓦林在小鼠试验中诱导了亡,由增加的Caspase3/9 mRNA和CASPASE9蛋白水平证明.
与自,DNA甲基化和RNA m6A甲基化 (包括关键酶和结合蛋白) 相关的基因表达显著减少.
RNA-seq确定了537个差异表达的基因,其中511个是下调的,影响各种信号通路. 通过RT-qPCR确认了几个关键基因的表达减少.

结论:

补充L-氨酸可以破坏小鼠丸的结构完整性.
观察到的丸损伤与诱导的亡和关键分子通路的失调有关,包括自和RNA甲基化.
这些发现凸显了氨酸过量对男性生殖健康的潜在不良影响,并要求进一步研究其机制.

关键词:

细胞灭亡 (apoptosis) 是一种死亡的过程.自自是一种自的过程.鼠标的丸是一个丸.核酸甲基化的核酸甲基化.l-氨酸的使用方法

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