循环二-GMP通过损害 Pseudomonas putida 中 NasT 的抗终结功能来抑制酸盐的同化
Liang Nie1, Yujie Xiao1, Tiantian Zhou1
1National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
Nucleic acids research
|November 24, 2023
概括
周期性迪加尼酸盐 (c-di-GMP) 调节细菌的酸盐同化. 高水平的c-di-GMP抑制了NasT与nirBDRNA的结合,降低了酸盐还原酶的活性,增加了酸盐的积累.
科学领域:
- 微生物生理学 微生物生理学
- 细菌的第二使者
- 酸盐的新陈代谢
背景情况:
- 循环基亚尼酸盐 (c-di-GMP) 是一种无处不在的细菌第二信使,控制细胞过程.
- 对于c-di-GMP在调节酸盐同化中的作用仍然基本未知.
- 纳斯是一种抗终结剂,对 Pseudomonas putida 中的酸盐同化至关重要.
研究的目的:
- 调查c-di-GMP在调节酸盐同化中的作用.
- 阐明c-di-GMP影响NasT功能的机制.
- 为了确定c-di-GMP,NasT和nirBD操作子之间的特定相互作用.
主要方法:
- 细菌遗传学和分子生物学技术.
- 用RNA结合测试来研究NasT-NalA相互作用.
- 位点定向的突变发生和分子对接以确定关键残留物.
- 对酸盐减少酶的酶活性测定.
主要成果:
- 纳斯特特特别结合了c-di-GMP.
- 高水平的c-di-GMP抑制了NasT与nirBD操作子领导RNA (NalA) 的结合.
- 这种抑制减弱了NasT的抗终止功能,降低了nirBD表达和酸盐还原酶活性,导致酸盐的积累.
结论:
- c-di-GMP通过抑制NasT. putida来负面调节P. putida中的酸盐同化.
- 在NasT中的特定残留物,特别是R140,对于c-di-GMP和Nala结合至关重要.
- 通过c-di-GMP介导的NasT调节在几个Pseudomonas物种中得到保留.
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