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碳素甲基化和TEMPO介导的氧化对多糖胺修饰的表征和比较

Wei Wang¹, Jiayuan Liu², Huiwen Xu²

¹State Key Laboratory of Marine Food Processing and Safety Control, College of Food Science and Engineering, Ocean University of China, Qingdao 266404, China; Qingdao Key Laboratory of Food Biotechnology, Qingdao 266404, China; Key Laboratory of Biological Processing of Aquatic Products, China National Light Industry, Qingdao 266404, China.

International journal of biological macromolecules

|November 24, 2023

概括

碳基甲基化比TEMPO氧化引入更多的碳基到诸如纤维素和素之类的多糖体中,而分子降解较少. 本研究比较了这些方法用于多糖改性.

科学领域:

生物化学生物化学
聚合物化学聚合物化学
材料科学材料科学材料科学

背景情况:

多糖是多功能生物聚合物,具有多种应用.
引入炭基组改变了聚糖的特性,使其适用于特定用途.
碳素甲基化和TEMPO氧化是多糖功能化的常见方法.

研究的目的:

为了比较碳素甲基化和TEMPO介导氧化在将碳素基引入多糖的效率.
评估这些修饰方法对多糖的分子量和结晶性的影响.
通过每种方法分析碳酸组引入的选择性.

主要方法:

模型中的多糖 (纤维素和基) 经过了碳氧甲基化.
模型多糖类也通过TEMPO介导的氧化方法进行了修改.
测量了修饰过的多糖类的碳基含量,分子量和结晶性.

主要成果:

与TEMPO氧化 (1.64 mmol/g纤维素,1.12 mmol/g基) 相比,碳酸甲基化产生了显著更高的碳酸基群含量 (4.99 mmol/g纤维素,4.46 mmol/g基).
时间氧化导致了大量的多糖降解,使分子量减少了42.46% (纤维素) 和64.5% ().
碳氧化甲基化降低了多糖类结晶度,增加了碳酸含量,而TEMPO氧化并没有改变结晶度.

结论:

碳基甲基化更有效地实现了多糖类中高碳基含量.
通过TEMPO介导的氧化导致了显著的多糖降解,并选择性地向C6基.
碳氧甲基化修改了所有基团,影响了结晶性,使其成为高功能的首选方法,而不会损害分子完整性.

关键词:

碳氧甲基化是碳氧甲基化的一种方法.聚糖的修饰方法是多糖.在TEMPO中介的氧化过程中.

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