相关实验视频

Updated: Jul 10, 2025

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Published on: March 11, 2020

在番茄中,Expansin SlExp1和内葡萄糖酶 SlCel2协同促进水果软化和细胞壁分解

Guanqing Su¹, Yifan Lin¹, Chunfeng Wang¹

¹College of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.

|November 24, 2023

概括

水果软化导致收获后损失. 科学家们使用CRISPR基因编辑来证明,同时淘汰西红中的SlExp1和SlCel2基因,通过调节细胞壁解体来增强水果坚硬度.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
生物化学生物化学

背景情况:

水果在成熟过程中变软,导致收获后的重大损失.
细胞壁的拆卸是减少水果坚度的主要驱动因素.
以前针对细胞壁基因的基因修改,除了乳酸酶,对坚性影响有限.

研究的目的:

研究番茄果实成熟相关的α-expansin (SlExpansin1/SlExp1) 和内葡萄糖酶 (SlCellulase2/SlCel2) 在细胞壁矩阵拆解和果实软化中的作用.
确定SlExp1和SlCel2对水果坚硬性和细胞壁完整性的协同作用.

主要方法:

利用CRISPR/CRISPR相关核酶9基因组编辑,在西红 (Solanum lycopersicum) 中创建单个并同时淘汰SlExp1和SlCel2.
评估果实坚硬度,细胞壁成分脱聚合 (pectin和xyloglucan) 和细胞粘附.
研究了SlExp1和SlCel2同时过度表达对水果软化的影响.

主要成果:

同时淘汰SlExp1和SlCel2显著提高了番茄果实坚硬度.
双重淘汰减少了同位素甲氨酸类型的pectin和xyloglucan的脱聚合,从而保持了细胞壁的完整性.
单个SlExp1或SlCel2的淘汰对果实坚硬度的影响很小,而它们同时过度表达则加速软化.

结论:

在番茄中,SlExp1和SlCel2协同作用,调节细胞壁解体和水果软化.
针对SlExp1和SlCel2提供了一种有希望的策略,可以提高收获后果的质量并减少浪费.

更多相关视频

OLIgo Mass Profiling OLIMP of Extracellular Polysaccharides

OLIgo Mass Profiling OLIMP of Extracellular Polysaccharides

Published on: June 20, 2010

Author Spotlight: Integrating Biochemical Functions of β-Glucanases and Peroxidase Enzymes in Wheat-RWA Interaction

Author Spotlight: Integrating Biochemical Functions of β-Glucanases and Peroxidase Enzymes in Wheat-RWA Interaction

Published on: July 26, 2024

相关概念视频

Role of Microtubules in Cell Wall Deposition

Role of Microtubules in Cell Wall Deposition

Microtubules are small hollow tubes in eukaryotic cells. The cell wall microtubules are polymerized dimers of two globular proteins, α-tubulin and β-tubulin, two globular proteins. With a diameter of about 25 nm, microtubules are the widest components of the cytoskeleton. They help the cell resist compression and provide a track along which vesicles move through the cell or pull replicated chromosomes to opposite ends of a dividing cell. Microtubules go through quick cycles of...

Cellulose and Pectic Polysaccharides

Cellulose and Pectic Polysaccharides

Every plant cell has a cell wall that protects the cell, provides structural support, and gives the cell shape. Cellulose, the main structural component of the plant cell wall, makes up over 30% of plant matter. It is the most abundant organic compound on earth. Cellulose is an unbranched polysaccharide composed of linear chains of glucose molecules linked by β (1→4) glycosidic bonds.
As a cell matures, its cell wall specializes according to its type. For example, the...