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The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
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使用基于蝶运动的光探测器探索菌体内深度依赖的微粘度

Xuanying Chen1, Shideng Yuan2, Mengyuan Qiao1

  • 1Key Laboratory for Advanced Materials and Feringa Nobel Prize Scientist Joint Research Center, Frontiers Science Center for Materiobiology and Dynamic Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.

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|November 24, 2023
PubMed
概括

研究人员开发了新的光表面活性剂 (DPAC-Fn) 来测量菌体内的粘度. 他们发现了从核到其表面的显著粘度变化,为细胞研究提供了新的工具.

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科学领域:

  • 超分子化学
  • 物理化学
  • 材料科学

背景情况:

  • 微粒内部呈现复杂的粘度梯度,很难通过实验来测量.
  • 了解这些梯度对于药物输送和催化中的应用至关重要.

研究的目的:

  • 开发和使用新型光表面活性剂来量化菌体内深度依赖的微粘度.
  • 调查表面活性剂链长度与内粘度之间的关系.

主要方法:

  • 通过使用粘度传感器 (DPAC) 功能化基三甲酸盐来合成基替代表面活性剂 (DPAC-Fn).
  • 使用DPAC-Fn对粘度敏感的多色辐射探测微粒环境.
  • 采用外部标准来测量化 (CTAB) 中的粘度.

主要成果:

  • 在CTAB中DPAC-Fn的浸泡深度随链长度 (n) 变化.
  • 观察到高效的,对粘度敏感的多色发射,与浸泡深度相关.
  • 在~4 nm CTAB 微粒中量化了显著的粘度梯度,从核心的~190 Pa·s到表面的~1 Pa·s.

结论:

  • 开发的DPAC-Fn表面活性剂是探索内微度的强大工具.
  • 这项研究通过实验证实并量化了从细胞中心到表面的显著粘度变化.
  • 这为深入的细胞表征提供了量身定制的方法.