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M1和M2巨细胞不同调节结肠密室的更新

Sathuwarman Raveenthiraraj^1,2, Griselda Awanis¹, Marcello Chieppa³

¹School of Pharmacy, University of East Anglia, Norwich Research Park, Norwich, Norfolk, NR4 7TJ, UK.

Inflammatory bowel diseases

|November 24, 2023

概括

炎症性M1巨细胞,但不是M2巨细胞,通过增加Lgr5+干细胞和通过直接接触的Wnt信号,促进结肠密室再生.

科学领域:

胃肠病学胃肠病学
免疫学免疫学免疫学
干细胞生物学干细胞生物学

背景情况:

大肠表皮通过Lgr5+肠干细胞快速更新,这对于对肠道微生物群的屏障功能至关重要.
炎症在密室附近引入免疫细胞,如M1巨细胞,但它们对密室再生的影响尚不清楚.

研究的目的:

研究M1和M2巨细胞对结肠密室再生和更新的不同影响.

主要方法:

利用体外巨细胞密码共同培养模型来研究细胞相互作用.
分析了密码细胞增殖,分化标志物和干细胞种群的变化.

主要成果:

无论是M1和M2巨细胞都增加了密码细胞的增殖;M2需要接触,M1通过分泌的因素起作用.
通过身体接触,M1巨细胞减少了杯状/状细胞和增加了Lgr5+干细胞.
通过直接接触,M1巨细胞增强了Wnt信号通路组件 (cyclin D1,LEF1).

结论:

不同的巨细胞亚型 (M1与M2) 对结肠密室生物学产生不同的影响.
M1巨细胞和密码表皮之间的直接细胞相互作用是干细胞扩张和炎症期间Wnt信号的关键驱动因素.

关键词:

一个Lgr55,一个Lgr5 结肠结肠结肠结肠是什么意思这是一种炎症炎症炎症炎症.巨细胞是什么?巨细胞是什么?干细胞是干细胞的组成部分.

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