来自蜜蜂肠道共生体的的鉴定,作为潜在的抗菌剂,用于对抗Melissococcus plutonius
Haoyu Lang1, Yuwen Liu1, Huijuan Duan1
1College of Food Science and Nutritional Engineering, China Agricultural University, 100083, Beijing, China.
Nature communications
|November 24, 2023
概括
蜜蜂肠道微生物拥有多样化的天然产品生物合成基因集群 (BGCs). 一些BGC,如Gilliamella中的那些,显示出作为抗菌剂对抗蜜蜂病原体的潜力.
科学领域:
- 微生物学 微生物学
- 化学生物学 化学生物学
- 昆虫科学 昆虫科学 昆虫科学
背景情况:
- 欧社会授粉者,包括蜜蜂和大蜂,拥有简单的肠道微生物群落,对宿主健康和病原体防御至关重要.
- 基因组挖掘的近期进展揭示了这些肠道微生物组内生物活性天然产品的生物合成途径,调解微生物-微生物相互作用.
研究的目的:
- 研究蜜蜂肠道微生物群中的生物合成基因集群 (BGCs) 的多样性和分布.
- 从蜜蜂共生体中发现具有潜在抗菌作用的新型天然产品.
主要方法:
- 分析了477种细菌基因组,包括从蜜蜂肠道微生物组中培养的分离物和元基因组组装的基因组.
- 生物合成基因集群 (BGCs) 的生物信息识别和表征.
- 用光谱指导的基因组挖掘来识别特定的BGC及其编码产品.
主要成果:
- 鉴定了744个生物合成基因集群 (BGCs) 在蜜蜂肠道微生物群中的多种化学类别.
- 发现基因集群分布在蜜蜂物种和地理位置之间存在显著差异,这与菌株水平的变化有关.
- 在Gilliamella菌株中鉴定了一种类似于 thiopeptide 的 BGC,该菌株对病原体Melissococcus plutonius.表现出强烈的活性.
结论:
- 蜜蜂肠道共生体有大量的小分子编码BGCs,有助于微生物群的化学多样性.
- 从蜂肠共生体中获得的细菌衍生的天然产品代表了潜在的抗微生物药物的有希望的来源,用于对抗蜜蜂疾病.
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