通过综合种群和泛基因组分析,深入了解家用的遗传多样性和表型变异
Guangliang Gao1,2,3, Hongmei Zhang4,5, Jiangping Ni6
1Chongqing Academy of Animal Science, Rongchang District, Chongqing, 402460, China.
Journal of animal science and biotechnology
|November 24, 2023
概括
这项研究构建了一个子泛基因组,识别了与身体重量和羽毛发育等特征相关的新型基因和选择信号. 该资源增强了对 domestication 的理解,并有助于未来的繁殖工作.
科学领域:
- 基因组学就是基因组学.
- 动物遗传学动物遗传学
- 进行比较基因组学.
背景情况:
- 家庭可以追溯到天 (Anser cygnoides) 或灰 (Anser anser) 的血统.
- 在种中,体型,繁殖,卵产量和羽毛颜色存在显著的表型变异.
- 了解遗传多样性对于改善品种至关重要.
研究的目的:
- 为人口基因组分析构建一个全面的全基因组.
- 识别基因变异,新基因和表型多样性的基础选择信号.
- 为未来的养和遗传研究提供基础资源.
主要方法:
- 在659只身上进行了全基因组再测序.
- 编制了155个RNA-seq样本.
- 全基因组构建和分析,包括核,软核,和云基因的识别.
- 使用SNP和存在缺席变异的全基因组关联研究 (GWAS).
- 在多种组织中分析基因表达模式.
主要成果:
- 产生了612 Mb的非参考结合物,确定了2813个新基因,并对15567个核心,1324个软核心,2734个shell和878个云基因进行了目录.
- 检测到含有TGFBR2的81.97 Mb选定基因组区域,与体重变化相关.
- GWAS揭示了SNP/存在缺席变化和包括肉质,繁殖和身体组成 (例如SVEP1基因和尸体斜长) 在内的特征之间的显著关联.
- 全基因组分析强调了在"毛囊成熟"中的可变基因的丰富,以及在养中"调节心脏收缩"基因的频率降低.
结论:
- 确定了许多选择信号和候选基因,这些基因对于了解的化和繁殖至关重要.
- 构建的子泛基因组提供了一个全面的基因组资源.
- 该资源提供了新的见解,并将对未来的繁殖计划做出重大贡献.
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