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草药化合物达瑞辛和伊索林辛阻碍了SARS-CoV-2病毒进入

Shaneek Natoya Dabrell¹, Yi-Chuan Li², Hirohito Yamaguchi^3,4

¹International Master's Program of Biomedical Sciences, China Medical University, Taichung 406040, Taiwan.

|November 25, 2023

概括

两种草药化合物,达瑞辛和伊索林辛,通过阻断Spike-ACE2相互作用,有效地抑制SARS-CoV-2的进入. 这些天然化合物显示出作为治疗病毒感染的治疗剂的前景.

科学领域:

病毒学病毒学
药物发现药物发现药物发现
分子生物学分子生物学

背景情况:

SARS-CoV-2 病毒的进入依赖于 Spike 蛋白与 ACE2 等宿主细胞受体的相互作用.
诸如TMPRSS2之类的蛋白酶通过分裂尖端蛋白质来促进病毒的进入.
针对Spike-ACE2相互作用是开发抗病毒疗法的关键策略.

研究的目的:

调查草药化合物杜利辛和伊索林辛在抑制SARS-CoV-2病毒进入方面的潜力.
阐明这些化合物影响病毒进入的机制,特别是Spike-ACE2相互作用和TMPRSS2活性.

主要方法:

利用表达SARS-CoV-2尖端蛋白的伪病毒进行感染分析.
评估了多利辛和伊索林辛对Spike-ACE2相互作用和TMPRSS2蛋白酶活性的影响.
采用分子建模来识别化合物与Spike蛋白之间的相互作用.

主要成果:

杜利辛和伊索林辛证明抑制了SARS-CoV-2病毒进入.
这些化合物降低了Spike-ACE2相互作用,但没有影响TMPRSS2蛋白酶活性.
分子建模揭示了杜利辛/伊索利因素和Spike蛋白的R403残留物之间的特定相互作用.

结论:

杜利辛和伊索林辛是通过向Spike-ACE2相互作用来有效抑制SARS-CoV-2进入的抑制剂.
这些草药化合物代表了治疗SARS-CoV-2感染的潜在治疗剂.
与Spike蛋白残留物R403的相互作用是这些化合物的抗病毒活性的一个关键因素.

关键词:

在 COVID-19 疫情中,这就是SARS-CoV-2病毒.流行病是一种流行病.疫苗,疫苗的使用情况.

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