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精液参数和人类精子微RNA资料的年龄相关变化

Joana Santiago¹, Joana V Silva¹, Manuel A S Santos^1,2

¹Department of Medical Sciences, Institute of Biomedicine (iBiMED), University of Aveiro, 3810-193 Aveiro, Portugal.

|November 25, 2023

概括

先进的父亲年龄 (APA) 可能会通过改变精子微RNA配置文件影响生育能力,即使传统的精液参数保持正常. 这表明microRNAs在老年男性的生殖结果中起着关键作用.

科学领域:

生殖生物学生殖生物学
遗传学遗传学是一个
衰老研究研究衰老研究

背景情况:

延迟育儿是一个日益增长的趋势,增加了晚年父亲年龄 (APA) 的发生率.
APA (男性>40岁) 与受损生育能力和不良生殖结果有关.
精子微RNA越来越多地被认为是它们在受精和胚胎发育中的作用.

研究的目的:

为了研究男性年龄对精液参数的影响.
分析与父亲年龄相关的精子微RNA概况.
确定潜在的分子机制,将APA与降低生育能力联系起来.

主要方法:

对333名葡萄牙男性 (2018-2022) 的射精进行分析,以寻找传统的精液参数.
在四个年龄组 (≤30,31-35,36-40,>40岁) 的16名诺莫动物精子男性中进行精子微RNA表达概况分析.
使用miRDB,TargetScan和DAVID工具对微RNA目标基因和途径进行生物信息分析.

主要成果:

男人的年龄和传统的精液参数之间没有显著的相关性,除了随着年龄的增长,射精量下降.
在各个年龄组中确定了15种差异表达的微RNA (DEM).
丰富分析显示,老年男性的DEM参与胚胎发育,形态发生和雄性腺发育,其目标与衰老途径 (衰老,自,胰岛素,mTOR) 有关.

结论:

传统的精液参数可能不完全反映男性衰老对生育能力的影响.
与晚年父亲年龄相关的精子微RNA概况的变化可能是减少生殖成功的基础.
精子微RNA代表了与年龄相关的生育能力下降的潜在生物标志物和治疗点.

关键词:

晚年父亲的年龄.生育能力生育能力生育能力生育能力这是一个微型RNA.精液参数精液参数

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