基于细胞系的人类膀有机体具有膀类的自我组织 - - 膀癌研究的新标准化方法
Mandy Berndt-Paetz1, Shanfu Han2, Annett Weimann1
1Department of Urology, Research Laboratories, Leipzig University, 04103 Leipzig, Germany.
Biomedicines
|November 25, 2023
概括
膀癌有机体模仿瘤结构和药物反应,为研究提供了有价值的模型. 这些三维模型有助于理解膀癌,并开发新的抗癌疗法.
科学领域:
- 在瘤学瘤学.
- 生物技术是生物技术.
- 癌症研究 癌症研究
背景情况:
- 三维 (3D) 器官对推动膀癌 (BCa) 研究至关重要.
- 由多种细胞类型组成的器官精确地复制了固体瘤的结构,增殖,细胞相互作用和药物反应.
研究的目的:
- 开发和描述人类BCa细胞系和膀侧膜细胞的新型3D器官模型.
- 评估这些有机体对研究BCa生物学和评估抗癌疗法的有用性.
主要方法:
- 使用人类BCa细胞系与膀纤维细胞和光滑肌肉细胞的四种器官的开发.
- 通过免疫活性对细胞激素,维丁,α-actin和KI67.67进行表征.
- 调查Wnt/β-catenin通路的激活和对多克索鲁比辛和放射治疗的反应.
主要成果:
- 器官体表现出类似膀壁的结构,其中包括尿细胞和支持细胞核心.
- 免疫活性和增殖率与BCa细胞起源相关;RT-112有机体显示上皮层分层.
- Wnt10B处理在高档有机体中激活了β-catenin. 多克索鲁比使生命能力降低了10-30%. 放射治疗的疗效因扩散状况而有所不同.
结论:
- 基于细胞系的有机体成功地重现了类似膀的结构和体内特征,包括尿分化.
- 这些有机体在膀癌研究中的功能研究中起到有效的作用.
- 开发的有机物对抗癌药物开发和个性化医疗方法充满希望.
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