通过蛋白质谷氨酸酶介导的除化,增强豆蛋白的溶解性和减少豆蛋白中的热聚合
Lijuan Luo1,2, Yuanyuan Deng1, Guang Liu1
1Sericultural & Agri-Food Research Institute Guangdong Academy of Agricultural Sciences, Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, China.
Foods (Basel, Switzerland)
|November 25, 2023
概括
蛋白质谷氨酸酶修饰显著改善了豆蛋白分离物的溶解性和热稳定性. 这种脱化过程增强了功能性质,使豆蛋白更适合于液体食品应用.
科学领域:
- 食品科学 食品科学 食品科学
- 蛋白质化学 蛋白质化学
- 生物化学 生物化学
背景情况:
- 豆蛋白分离物 (PPI) 的溶解性和稳定性有限,限制了它们在液体配方中的使用.
- 酶修饰提供了一个潜在的策略来克服这些局限性.
研究的目的:
- 研究蛋白质谷氨酸酶 (PG) 修饰对豆蛋白分离物 (PPI) 功能性质的影响.
- 阐明在非甲基化 PPI 中提高溶解度和热稳定性的潜在机制.
主要方法:
- 使用蛋白质谷氨酸酶去氨基化豆蛋白分离物,以达到不同程度的去氨基化 (DD, 10-25%).
- 通过可溶性和热稳定性测试来评估功能性质.
- 用SDS-PAGE,泽塔潜力,FTIR,表面水性和内在光来分析结构变化.
- 使用高性能大小排除色谱和传输电子显微镜研究了聚合行为.
主要成果:
- 修改PG显著提高了PPI的可溶性和热稳定性,特别是在DD>20%.
- 脱化增加了PPI净电荷,促进了蛋白质的展开,暴露了疏水性群体.
- 增加的静电排斥促进了蛋白质与水的相互作用,并阻碍了在高温下聚合.
结论:
- 通过PG介导的除是一种有前途的方法,可以改善豆蛋白分离物的功能性质.
- 优化的除化可以提高PPI适用于各种基于液体的食品应用的适用性.
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