在Spike RBD的结合部位的低缩水可能会揭示SARS-CoV-2变种的传染性
Lin Yang1,2, Shuai Guo1, Chengyu Hou3
1National Key Laboratory of Science and Technology on Advanced Composites in Special Environments, Center for Composite Materials and Structures, Harbin Institute of Technology, Harbin 150080, China.
Biomolecules
|November 25, 2023
概括
SARS-CoV-2 变种与人类 ACE2 受体的结合亲和力是由形状相匹配的低的水合驱动的. 这种涉及疏水吸引力的机制有助于估计病毒的传染性和传染性.
科学领域:
- 病毒学 病毒学
- 结构生物学 结构生物学
- 生物物理学的生物物理.
背景情况:
- 严重急性呼吸道综合征冠状病毒2 (SARS-CoV-2) 感染性依赖于与ACE2受体结合的尖端蛋白受体结合域 (RBD).
- 了解这种相互作用对于开发有效的抗病毒策略至关重要.
研究的目的:
- 为了研究水化和在SARS-CoV-2 RBD-ACE2结合亲缘关系中的作用.
- 为了确定病毒感染性和传染性背后的生物物理机制.
主要方法:
- 在蛋白质表面上选伪水友群.
- 在ACE2和SARS-CoV-2 RBDs的水化中分析低的区域.
- 形状匹配分析以确定结合机制.
主要成果:
- 在ACE2和多个SARS-CoV-2变种的水化上展示了低区的分布.
- 确定了低的水解的形状匹配是疏水吸引力和结合亲和力的关键驱动因素.
- 已确立的低水化状况作为传染性的衡量标准.
结论:
- SARS-CoV-2 RBD-ACE2 的结合是由与形状相匹配的低度水化之间的疏水性崩控制的.
- 在尖端蛋白结合部位的水化的低度水平是病毒传染性的指标.
更多相关视频
08:07A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain
Published on: July 25, 2022
2.6K
08:40Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting
Published on: March 1, 2019
59.0K
相关概念视频
02:28Leaky Scanning
5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K
02:50Conjugated Proteins
18.3K
Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
18.3K
02:35Noncovalent Attractions in Biomolecules
51.4K
Noncovalent attractions are associations within and between molecules that influence the shape and structural stability of complexes. These interactions differ from covalent bonding in that they do not involve sharing of electrons.
Four types of noncovalent interactions are hydrogen bonds, van der Waals forces, ionic bonds, and hydrophobic interactions.
Hydrogen bonding results from the electrostatic attraction of a hydrogen atom covalently bonded to a strong-electronegative atom like oxygen,...
Four types of noncovalent interactions are hydrogen bonds, van der Waals forces, ionic bonds, and hydrophobic interactions.
Hydrogen bonding results from the electrostatic attraction of a hydrogen atom covalently bonded to a strong-electronegative atom like oxygen,...
51.4K