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迪西利醇影响不成熟和成熟的海星卵细胞的受精反应

Nunzia Limatola¹, Jong Tai Chun², Kazuyoshi Chiba³

¹Department of Research Infrastructures for Marine Biological Resources, Stazione Zoologica Anton Dohrn, 80121 Napoli, Italy.

|November 25, 2023

概括

迪三醇 (DTT) 通过改变皮质活动来减少海星卵细胞中的多精子. 然而,与1-甲基氨酸 (1-MA) 不同,DTT 不会诱导胚芽囊泡分解 (GVBD),损害受精和发育.

科学领域:

海洋生物学海洋生物学
发展生物学发展生物学
细胞生物学细胞生物学

背景情况:

未成熟的海星卵细胞易患多精子症,原因是维他林层结构和皮质活动.
由1-甲基氨酸 (1-MA) 触发的卵细胞成熟,涉及活性蛋白重塑和生殖囊泡分解 (GVBD) 进行正常受精.
低硫化物降解剂,如滴硫醇 (DTT),可以诱导卵细胞成熟和GVBD在一些海星物种.

研究的目的:

研究dithiothreitol (DTT) 对*Astropecten aranciacus*卵细胞中受精反应的影响.
为了比较DTT与1-甲基氨酸 (1-MA) 在诱导卵细胞成熟和受精能力方面的作用.

主要方法:

用DTT处理未成熟的*Astropecten aranciacus*卵细胞,有或没有1-MA.
对受精率,精子诱导的 (Ca2+) 反应和卵细胞形态 (微,皮质颗粒,F-actin) 的分析.
在DTT治疗后评估胚芽囊泡分解 (GVBD).

主要成果:

短时间的DTT治疗降低了A. aranciacus*卵细胞中的多精子受精率.
通过修改微,皮质颗粒和皮质F-actin,DTT改变了精子诱导的Ca2+反应.
DTT治疗 (70分钟) 与1-MA不同,没有诱导GVBD,并且导致F-actin脱聚合,损害受精和发育.

结论:

DTT通过改变皮质动蛋白和微粒细胞,减少多精子症,但也损害了正常的受精过程,从而影响海星卵细胞受精.
DTT诱导的F-actin脱聚合与1-MA诱导的成熟不同,突出了实现受精条件的不同途径.
虽然DTT可以减轻多精子症,但它破坏了成功受精和随后胚胎发育的必要机制.

关键词:

在 DTT 上,你会看到 DTT.这就是Actin Actin.和是最重要的.减少二硫化物的降解剂.这是受精,受精,受精.卵细胞成熟的过程多种精子多种性.海星是什么意思海星是什么意思

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