应用方法评估金属基纳米颗粒的抗菌活性
Etelka Chung1, Guogang Ren1, Ian Johnston1
1Centre for Engineering Research, University of Hertfordshire, Hatfield AL10 9AB, UK.
Bioengineering (Basel, Switzerland)
|November 25, 2023
概括
针对抗微生物纳米材料的标准化测试是至关重要的,因为抗生素耐药性日益增加. 井扩散方法在确认活性方面最可靠,而复素稀释确定最小抑制度 (MIC).
科学领域:
- 抗微生物耐药性研究的研究.
- 纳米材料的安全性和有效性
- 在体外诊断在体外诊断.
背景情况:
- 抗生素耐药性需要新的抗菌药物发现和标准化测试.
- 对水性试剂的现有方法已经建立,但测试纳米材料等基于颗粒的悬浮物仍然具有挑战性.
- 对纳米材料测试的ISO标准验证已经面临审查,需要采取纠正措施.
研究的目的:
- 评估五种体外测试,以测试金属基纳米材料的抗菌活性.
- 评估每个测试方法的可靠性和局限性.
- 确定确认抗微生物活性和确定纳米材料最小抑制度 (MIC) 的最佳方法.
主要方法:
- 使用五种不同的体外测定方法对10种金属基纳米材料对10种病原体进行序列分析.
- 评估每个测试的技术,局限性和稳定性.
- 艾格尔井扩散和雷萨苏林稀释方法的有效性比较和MIC确定.
主要成果:
- 至少需要两种方法来确认金属基纳米材料悬浮物的抗菌活性.
- 阿加井扩散方法是最可靠的,提供了关于疗效,离子和协同效应的数据.
- 推用resazurin稀释剂来确定MIC,提供可视化结果并克服检测问题.
- 双金属AgCu显示出最高的抗微生物有效性 (细菌MIC为7μg/mL,真菌MIC为62.5μg/mL).
结论:
- 标准化和验证的体外测定对于评估抗微生物纳米材料至关重要.
- 阿加尔井扩散和雷萨苏林稀释的组合提供了全面的评估.
- AgCu双金属纳米颗粒显示出作为抗菌剂的巨大潜力.
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