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尼古丁,THC和多卢特格拉维尔调节电子烟诱导的成和炎症相关基因的变化,在老鼠大脑和星球细胞中

Jacqueline Renee Kulbe¹, Lauren Nguyen¹, Alexandra Anh Le¹

¹Department of Psychiatry, University of California San Diego, La Jolla, CA 92093, USA.

|November 25, 2023

概括

电子烟的溶剂乙烯甘醇 (PG) 改变了大脑的基因表达,潜在地提供了对尼古丁和大麻依赖的保护. 然而,PG也可能引起神经炎症,特别是对于使用某些抗逆转录病毒药物的人来说.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
药理学药理学是指药理学的学科.

背景情况:

电子烟的使用在艾滋病毒感染者 (PWH) 中普遍存在,并被销售为比传统吸烟更安全.
早期生长受体2 (EGR2) 和活动调节的细胞骨相关蛋白 (ARC) 与成,神经炎症和神经退行有关.
与艾滋病毒相关的神经认知障碍是PWH的一个重大问题.

研究的目的:

为了研究电子烟蒸汽成分对大脑基因表达的影响.
探索甘醇 (PG) 的潜在神经保护或神经炎症作用及其与尼古丁 (NIC),THC和HIV抗逆转录病毒药物 (DTG) 的相互作用.

主要方法:

暴露Sprague Dawley大鼠在电子烟蒸汽中的10天时间.
使用培养的C6大鼠星体细胞进行了体外研究.
在脑组织和星球细胞中对EGR2和ARCmRNA表达的分析.
研究PG,NIC,THC和DTG对基因表达的影响.

主要成果:

在大鼠前皮层中,PG降低了EGR2和ARCmRNA的调节,这种效应被NIC和THC逆转.
在体外,PG在星球细胞中调节了EGR2和ARCmRNA,这表明神经炎症的潜力.
NIC逆转了星球细胞中PG诱导的上调,但THC没有.
艾滋病毒抗逆转录病毒DTG逆转了NIC对PG诱导的EGR2上调调节的影响.

结论:

电子烟溶剂PG可能具有双重作用,可能保护NIC/THC依赖,但也诱导神经炎症.
由于EGR2在HIV潜伏期,T细胞亡和神经炎症中的作用,PG,NIC和DTG之间的相互作用对PWH是令人担忧的.
需要进一步的研究来了解PWH中电子烟使用的长期神经后果,特别是关于抗逆转录病毒相互作用.

关键词:

在这里,我们可以看到THCTHCTH.抗逆转录病毒药物抗逆转录病毒药物神经炎症是一种神经炎症.尼古丁是一种尼古丁.

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