相关实验视频

Updated: Jul 10, 2025

Novel Sequence Discovery by Subtractive Genomics

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

一种Prinsepia uniflora (科) 的高质量参考基因组组合

Lei Zhang¹, Chaopan Zhang¹, Yajing An¹

¹Key Laboratory of Ecological Protection of Agro-Pastoral Ecotones in the Yellow River Basin, National Ethnic Affairs Commission of the People's Republic of China, College of Biological Science & Engineering, North Minzu University, Yinchuan 750021, China.

|November 25, 2023

概括

一种药用草药Prinsepia uniflora的高质量基因组组装揭示了它的遗传构成. 这种基因组资源有助于理解它的进化及其更广泛的罗萨属植物家族.

科学领域:

基因组学就是基因组学.
植物科学植物科学
生物信息学是一种生物信息学.

背景情况:

普林塞皮亚单花 (Prinsepia uniflora) 是一株传统的中国药用草本,属于科植物.
高质量的基因组组合对于理解植物遗传学和进化至关重要.

研究的目的:

为了生成Prinsepia uniflora的染色体级基因组组合.
为了注释基因组,识别基因,重复元素和非编码RNA.
为了研究P. uniflora在Rosaceae家族中的进化历史.

主要方法:

染色体层次的基因组组装使用先进的测序和组装技术.
重复的序列注释,包括可转换的元素.
蛋白质编码基因预测和功能注释.
非编码RNA的识别.
遗传学分析以推断进化关系.

主要成果:

在16个伪染色体中实现了1272.71 Mb的基因组组装.
重复序列占基因组的61.07%,可转移的元素占主导地位.
确定了49261个编码蛋白质的基因,其中91.87%的功能注释.
发现了3080种非编码RNA.
遗传学分析表明,在P. uniflora的进化过去,最近发生了一次全基因组复制事件.

结论:

高质量的基因组组合为Prinsepia uniflora提供了宝贵的基因组资源.
这种组合将促进对物种生物学,药用特性和进化轨迹的进一步研究.
这些发现有助于罗萨ceae家族中的比较基因组学和进化研究.

关键词:

这是PacBio高保真测序.普林塞皮亚单花 (Prinsepia uniflora) 是一个植物.在染色体层面的基因组组合.基因组注释基因组注释药用植物药用植物

更多相关视频

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Published on: March 8, 2018

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

相关概念视频

Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.