Osa-miR11117 目标 OsPAO4 调节大米免疫力对抗爆发性真菌 Magnaporthe oryzae 的免疫力
Shang Gao1, Yan Hou1, Qiwei Huang1
1National Plant Space Breeding Engineering Technology Research Center, Guangdong Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou 510642, China.
International journal of molecular sciences
|November 25, 2023
概括
一种米的微RNA,Osa-miR11117,调节了对爆发性真菌Magnaporthe oryzae的免疫力. 准OsPAO4,它影响聚胺代谢和乙烯信号,影响爆电阻力.
科学领域:
- 植物科学 植物科学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 米对Magnaporthe oryzae的免疫是复杂的.
- 微RNA在植物防御中起着至关重要的作用.
研究的目的:
- 调查Osa-miR11117对抗米爆炸的作用.
- 确定 Osa-miR11117 的目标和监管途径.
主要方法:
- 干环qRT-PCR用于microRNA表达分析.
- 基于GFP的记者测定用于目标验证.
- qRT-PCR用于基因表达分析.
主要成果:
- 在M. oryzae感染时,osa-miR11117的表达增加.
- Osa-miR11117的过度表达会降低爆炸阻力.
- OsPAO4 是 Osa-miR11117.7 的一个直接目标.
- OsPAO4调节聚胺水平和乙烯信号传递.
结论:
- Osa-miR11117的目标是OsPAO4,以调节大米的爆炸阻力.
- Osa-miR11117/OsPAO4轴影响聚胺代谢和乙烯信号传递.
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