通过标记器辅助逆交繁殖对巴斯马提大米生物应激抵抗的遗传增强
Gagandeep Singh1, Niraj Singh1, Ranjith Kumar Ellur1
1Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India.
International journal of molecular sciences
|November 25, 2023
概括
新的巴斯马提大米品种 (普萨3037,3054,3060,3066,3122,3123,3124) 通过将细菌病菌和抗爆基因纳入普萨巴斯马提1509中来开发. 这些改进的米线提供了增强的抗病能力,并保持了出色的质量.
科学领域:
- 植物育种与遗传学
- 农业科学 农业科学
- 分子生物学分子生物学
背景情况:
- 普萨巴斯马蒂1509 (PB1509) 是一种高收益的巴斯马蒂大米品种,以其质量而闻名,但易受细菌爆发和爆发.
- 结合抗性基因对于提高作物弹性和减少由于生物压力的产量损失至关重要.
研究的目的:
- 开发改进的巴斯马蒂大米品种,增强对细菌病菌和爆炸的抵抗力.
- 为了保持重复的母体PB1509的可取的农业学,谷物和质量特征.
主要方法:
- 侵入性杂交被用于将细菌抗炎基因 (xa13 + Xa21, Xa38) 和抗炎基因 (Pi9 + Pib, Pita) 转移到PB1509.
- 使用基因链接标记的前景选择和使用简单序列重复 (SSR) 标记和80K SNP数组的背景选择.
- 接近同位素线 (NIL) 的发展和随后的精英线交叉结合多个抗性基因.
主要成果:
- 四个NILs (Pusa 3037,Pusa 3054,Pusa 3060,Pusa 3066) 被开发出来,与PB1509具有很高的基因组相似性 (>97%),携带特定的耐药基因.
- 通过GGE-biplot分析确定了稳定和表现更好的线路.
- 产生了三重基因金字塔线 (Pusa 3122, 3124, 3123),表现出与PB1509相比的农学和品质.
结论:
- 开发的巴斯马蒂大米品种具有对细菌病变和爆炸的增强抵抗力,确保减少杀虫剂的使用和提高生产率.
- 这些改良品种为农民提供了可持续的解决方案,改善了种植经济和消费者安全.
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