通过在玉米原生质中引入由聚合物和核酸组成的纳米粒子复合物引入CRISPR/Cas9突变
Bettina Nagy1, Ayşegül Öktem2,3, Györgyi Ferenc1
1Agribiotechnology and Precision Breeding for Food Security National Laboratory, Institute of Plant Biology, HUN-REN Biological Research Centre, 6726 Szeged, Hungary.
International journal of molecular sciences
|November 25, 2023
概括
这项研究优化了一种阴离子聚合物,聚-2-基胺 (PHPI),用于将基因编辑工具送入玉米细胞. 在植物原生质中,PHPI能够有效地进行CRISPR/Cas9基因编辑,从而促进作物改进.
科学领域:
- 植物科学 植物科学
- 生物技术是生物技术.
- 遗传学 是一个遗传学.
背景情况:
- 向基因突变发生对于植物研究和作物改良至关重要.
- 将基因编辑组件有效地输入植物细胞是一个关键的挑战.
研究的目的:
- 优化将基因编辑组件输送到玉米原生质中,使用化聚合物聚2-基烯胺 (PHPI).
- 为了证明在玉米细胞中进行CRISPR/Cas9基因编辑的PHPI介导输送的有效性.
主要方法:
- 在PHPI介导下,可塑性DNA和光标记的寡核酸被输入到玉米原生质中.
- 同时提供GFP表达性等离子体和寡核酸来评估局部化.
- 在转基因玉米原生体中使用PHPI复合体等离子体对EGFP基因进行CRISPR/Cas9基因编辑.
- 通过缺乏GFP光来识别编辑的细胞,并通过桑格测序进行确认.
主要成果:
- PHPI有效地将等离子体DNA和寡核化物输送到玉米原生质中.
- 成功的联合交付导致了GFP的细胞质和核积累以及寡核酸的优先核定位.
- 通过CRISPR/Cas9介导的EGFP基因的淘汰在玉米原生质中实现.
- 桑格测序证实了目标基因中的多基和单基删除.
结论:
- 性聚合物PHPI有效地将基因编辑组件输送到植物细胞中.
- 以PHPI为媒介的传递促进了玉米原生质中高效的CRISPR/Cas9基因组编辑.
- 这种方法有望促进植物研究和作物改进战略的发展.
关键词:
这就是CRISPR/Cas9的作用.这是一种DNA寡核酸.Ds红色光标记蛋白质在GFP表达式中,GFP表达式玉米原塑是玉米的原塑.突变发生的突变发生.纳米粒子是一种纳米粒子.质粒吸收的时间聚 (2-基烯 imine) 是一种多 (2-基烯 imine) 的物质.多重复合体是多重复合体.更多相关视频
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