在Plutella xylostella中,HSP70和HSP90精确调节RNAi效率
Sujie Lin1,2,3,4, Jie Yang1,2,3,4, Weiqing Wang1,2,3,4
1State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
International journal of molecular sciences
|November 25, 2023
概括
热冲击蛋白 (HSP) 在Plutella xylostella中的RNA干扰 (RNAi) 中至关重要. 抑制Hsc70-4和Hsp90降低了RNAi的效率,突出了它们在这种途径中的重要作用.
科学领域:
- 分子生物学分子生物学
- 昆虫科学 昆虫科学 昆虫科学
- 生物化学 生物化学
背景情况:
- 热冲击蛋白 (HSP) 在真核RNA干扰 (RNAi) 中起着分子陪伴的作用.
- 众所周知,HSP70和HSP90有助于模型生物中的阿尔戈诺特蛋白折叠.
- 在Plutella xylostella (钻石背) 的RNAi通路中HSP的特定作用尚不清楚.
研究的目的:
- 在Plutella xylostella中识别和描述PxHsc70-4和PxHsp83.
- 为了研究这些HSP在Plutella xylostella的RNA干扰途径中的功能.
- 为了阐明HSP对这种类昆虫RNAi效率的影响.
主要方法:
- 对PxHsc70-4和PxHsp83编码序列的序列分析.
- 在试验室中,针对PxHsc70-4和PxHsp83.3的淘汰和抑制实验.
- 在体内抑制试验和对记者基因的基因表达分析.
- 基于细胞的测定涉及DBM,Sf9和S2细胞中PxHsc70-4和PxHsp83的过度表达.
主要成果:
- PxHsc70-4和PxHsp83具有HSP特征的保存域.
- 抑制PxHsc70-4和PxHsp83显著降低了RNAi效率,由增加的记者基因表达 (PxmRPS18) 证明.
- 过度表达PxHsc70-4和PxHsp83也降低了各种细胞系中的RNAi效率.
结论:
- 特定量的Hsc70-4和Hsp83对于Plutella xylostella中的siRNA途径至关重要.
- 这些HSP在类昆虫的RNAi效率调节中起着至关重要的作用.
- 这些发现为昆虫RNAi通路中HSP的功能提供了新的见解.
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