在猪的多种组织中对circRNA表达特征进行全面分析
Qingpeng Shen1, Wentao Gong1, Xiangchun Pan1
1Guangdong Laboratory of Lingnan Modern Agriculture, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Swine and Poultry Breeding Industry, Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
International journal of molecular sciences
|November 25, 2023
概括
这项研究全面分析了九种猪组织中的循环RNA (circRNA),确定了数千种新型,家政和组织特异性的circRNA. 这些发现为猪基因表达和生物过程提供了关键的见解.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 动物科学动物科学
背景情况:
- 循环RNAs (circRNAs) 是基因表达的关键调节者,在猪发育中具有已知的作用.
- 之前对猪圈RNA的研究仅限于少数组织,需要进行更广泛的调查.
研究的目的:
- 在九种不同的猪组织中对circRNA进行全面分析.
- 识别新型,家政和组织特异性的circRNA并探索它们的功能.
- 通过网络分析和跨物种比较,研究circRNAs在猪生物学中的调控作用.
主要方法:
- 分析了来自9个猪组织的215个公开可用的RNA测序 (RNA-seq) 数据集.
- 鉴定和分类circRNAs,包括新型,家政和组织特定类型.
- 权重基因共同表达网络分析 (WGCNA) 和circRNA-miRNA-mRNA网络构建.
- 与人类对应物相比,猪肉环RNA的保护分析.
主要成果:
- 鉴定了82,528个circRNAs,包括3,818个新型,以及492个家庭和3,489个组织特异性的circRNAs.
- 丰富分析揭示了基本细胞过程中的家政circRNA和专门功能 (如肌肉,垂体,卵巢) 的组织特异性circRNA.
- WGCNA确定了14个模块,其中有1377个枢纽circRNA;网络分析阐明了circRNA,miRNA和mRNA之间的相互作用.
- 保存分析显示,19.29%的猪环RNA在人类中具有同类位置.
结论:
- 本研究介绍了猪肉环RNAs的广泛目录,突出了它们的组织特异性作用和调节潜力.
- 这些发现为了解猪基因表达,发育和进化生物学提供了宝贵的资源.
- 鉴定的circRNA及其网络为研究猪遗传学和功能基因组学提供了新的途径.
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