全转录组RNA测序揭示了子胚胎肌体发生的全球表达变化和RNA调节网络
Shuibing Liu1,2, Jintao Wu1,2, Wentao Zhang1,2
1College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China.
International journal of molecular sciences
|November 25, 2023
概括
这项研究揭示了编码和非编码RNA在子肌肉发育 (肌体发生) 中的复杂作用. 关键发现突出了糖脂代谢途径和调节这一关键过程的特定基因/miRNA.
科学领域:
- 动物科学动物科学
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 肉是重要的蛋白质来源,使的肌肉发育 (肌体生成) 对于营养很重要.
- 在子肌生成中编码和非编码RNA的调节网络尚未得到充分理解.
研究的目的:
- 为了研究参与子肌发生的RNA的表达模式和分子机制.
- 构建调节网络并确定子肌肉发育中的关键基因和途径.
主要方法:
- 全转录组RNA测序 (RNA-seq) 用于对mRNA,lncRNA,circRNA和miRNA进行分析.
- 进行了生物信息分析,包括基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG).
- 竞争的内源RNA (ceRNA) 网络被构建以确定调控相互作用.
主要成果:
- 数以千计的差异表达 (DE) mRNAs,lncRNAs,circRNAs和miRNAs在髓细胞和髓管之间被确定.
- 确定的关键途径包括铁,PPAR信号传递和糖脂代谢.
- 特定的lncRNAs,miRNAs (例如,miR-129-5p,miR-133a-5p) 和mRNAs (例如,ENSAPLG00020001677) 参与了肌体发生调节,特别是在与糖脂代谢相关的ceRNA网络内.
结论:
- 这项研究提供了关于子肌体发生的mRNA-lncRNA-circRNA-miRNA调节网络的全面资源.
- 糖脂代谢是子肌肉发育中的重要途径,由特定的分子参与者调节.
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