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脂肪衍生性介质干细胞 (MSC) 不朽化通过调节hTERT和TP53表达水平

Aigul R Rakhmatullina¹, Rimma N Mingaleeva¹, Dina U Gafurbaeva¹

¹Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, Russia.

Journal of personalized medicine

|November 25, 2023

概括

研究人员使用人类端粒酶逆转录酶 (hTERT) 和TP53敲击来使脂肪衍生中介质干细胞 (MSC) 不朽化. 由此产生的不朽化MSC (iMSC) 保持了差异化潜力,并增强了再生医学应用的扩散.

科学领域:

细胞生物学细胞生物学
再生医学是一种再生医学.
干细胞疗法是一种干细胞疗法.

背景情况:

介质细胞干细胞 (MSC) 对于组织修复至关重要,但在体外培养寿命和分化潜力有限.
现有的永生化方法可能会改变MSC的分化能力,并导致衰老.
脂肪酸衍生MSC对再生医学具有前景,但需要克服培养限制.

研究的目的:

建立一个基因稳定的,不朽的脂肪衍生MSC线,保持差异化潜力.
为了克服长期细胞治疗应用的初级MSCs的局限性.
调查hTERT过度表达和TP53敲击对MSC特征的影响.

主要方法:

主要脂肪衍生的MSCs通过过度表达人类端粒酶逆转录酶 (hTERT) 基因而被永生化.
与此同时,TP53基因表达被击倒,以增强永生.
由此产生的不朽化MSCs (iMSCs) 培养了30多个通道,并对差异化,增殖,形态和自我更新进行了评估.

主要成果:

不朽化MSCs (iMSCs) 在30个通道中保持了稳定的骨质生殖和肌体生殖分化潜力.
iMSCs表现出增加的细胞增殖率和增强的脂肪生成差异化潜力.
长期培养不影响iMSC形态或自我更新能力,建立基因稳定的细胞系.

结论:

通过hTERT过度表达和TP53淘汰的联合策略,成功地产生了稳定,不朽的脂肪衍生的MSC线 (iMSCs).
这些iMSC保留了关键的干细胞特性,包括多能性和自我更新,使它们适合再生医学.
这项研究通过克服体外培养局限性,为推进基于MSC的治疗策略提供了有价值的工具.

关键词:

在MSC中,MSC是MSC.在MSC区分的区分.永生化就是永生化.中介细胞干细胞中介细胞干细胞.瘤瘤瘤瘤

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