库尔库通过CXCL12/CXCR4轴扰乱了乳腺瘤微环境中的ADMSC和癌细胞之间的积极反循环
Bo-Young Jang1, Min Kyoung Shin1, Dong-Hee Han1
1Department of Life Science, Dongguk University-Seoul, Goyang 10326, Republic of Korea.
Pharmaceutics
|November 25, 2023
概括
黄素通过抑制CXCL12/CXCR4轴来破坏乳腺瘤微环境 (TME). 这阻断了脂肪衍生性介质干细胞 (ADMSC) 和癌细胞之间的相互作用,防止了癌症的进展.
科学领域:
- 在瘤学瘤学.
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 脂肪组织显著影响乳腺癌的发病和进展.
- 脂肪基衍生性介质干细胞 (ADMSC) 是乳腺瘤微环境 (TME) 的关键组成部分,与癌细胞相互作用.
- CXCL12/CXCR4轴介导ADMSC分化成癌症相关纤维细胞 (CAF),并促进癌细胞转移.
研究的目的:
- 为了研究黄素对乳腺TME的影响.
- 为了确定黄素是否可以破坏ADMSC和乳腺癌细胞之间的积极反循环.
- 分析黄素对CXCL12/CXCR4轴和下游信号传导的影响.
主要方法:
- 利用MCF7乳腺癌细胞条件介质 (MCF7-CM) 诱导ADMSC转化.
- 评估了黄素对ADMSC表型变化和CAF标志物表达的影响.
- 在ADMSC和MCF7细胞中测量了CXCL12/CXCR4轴和下游信号的抑制.
- 评估了用黄素治疗的ADMSC受条件介质对癌细胞反循环和上皮-介质细胞过渡的影响.
主要成果:
- 黄素减少了ADMSC表型变化,并抑制了CAF标记物表达.
- 黄抑制了CXCL12/CXCR4轴及其在ADMSC和MCF7细胞中的下游信号传递.
- 来自黄素治疗的ADMSC的条件介质抑制了ADMSC和MCF7细胞之间的正反循环.
- 库尔库明治疗抑制了MCF7细胞中的表皮层-介质细胞过渡.
结论:
- 黄素是重塑乳腺TME的强有力的药物.
- 黄素有效地限制了通过CXCL12/CXCR4轴介导的ADMSC癌症正反循环.
- 这些发现凸显了黄素作为一种针对瘤微环境的抗癌治疗策略的潜力.
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