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表面水中的抗微生物耐药性和毒性E. 大肠杆菌

Connor D LaMontagne¹, Elizabeth C Christenson^1,2,3, Anna T Rogers^4,5

¹Department of Environmental Science and Engineering, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, NC 27516, USA.

|November 25, 2023

概括

像大肠杆菌这样的环境细菌中的抗菌素耐药性 (AMR) 与毒性基因有负面关联. 这表明,耐药菌株可能在地表水中具有较低的病原性风险.

科学领域:

环境微生物学环境微生物学
抗微生物耐药性 (AMR) 是一种
病原体的毒性病原体的毒性

背景情况:

越来越多地认识到环境在抗菌素耐药性 (AMR) 的出现和传播中的作用.
了解环境耐药细菌的致病性对于公共卫生风险评估至关重要.
现有的关于AMR和毒性关联的研究是矛盾的,因为健身成本和遗传因素.

研究的目的:

调查抗生素耐药性和环境中的病毒性基因流行率之间的关系 Escherichia coli.
为了比较从表面水中与或没有商业猪操作 (CHOs) 的分离物中毒性基因的流行率.

主要方法:

使用了来自北卡罗来纳州地表水中的埃舍里希亚大肠杆菌的 AMR 异形特征.
隔离物根据对任何抗生素的耐药性和完全敏感性进行配对,并根据样本的日期和地点进行匹配.
传统的PCR被用来检测七个特定的毒性基因 (bfp, fimH, cnf-1, STa, EAST-1, eae, hlyA).

主要成果:

病毒性基因fimH的流行率很高 (93.1%).
除了fimH外,24.7%的分离物携带至少一种其他毒性基因.
在抗生素耐药性 (整体和四环素特异性) 与毒性基因存在 (整体和STa特异性) 之间观察到显著的负面关联.

结论:

对抗生素耐药的环境大肠杆菌表现出病毒性基因的减少.
这表明了潜在的权衡,在这种环境背景下,AMR可能与较低的病原性有关.
需要进一步的研究来探索与商业猪业务和其他环境因素的关联.

关键词:

美国大肠杆菌 (E. coli).抗微生物耐药性 (AMR) 是一种商业动物农业商业动物农业表面的水表面的水毒性毒性是一种毒性.

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