从婴儿配方加工设施中Cronobacter sakazakii隔离的综合基因组表征,使用全基因组测序
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Zeinab Ebrahimzadeh Mousavi1,2,3, Leonard Koolman1, Guerrino Macori1
1UCD-Centre for Food Safety, School of Public Health, Physiotherapy and Sports Science, University College Dublin, D04 V1W8 Dublin, Ireland.
Microorganisms
|November 25, 2023
概括
婴儿配方植物中Cronobacter sakazakii菌株显示主导的序列类型1和广泛的抗生素耐药性. 这种基因组研究有助于预防食物传播疾病的爆发.
科学领域:
- 食品安全微生物学 食品安全微生物学
- 基因组流行病学基因组流行病学
- 公共卫生微生物学
背景情况:
- 克罗诺巴克特 (Cronobacter sakazakii) 是一种机会性病原体,会导致婴儿脑膜炎等严重感染,通常与婴儿配方奶粉 (PIF) 相关.
- 了解C. sakazakii的遗传构成和抗生素耐药性概况对于防止食品生产环境中的疫情至关重要.
研究的目的:
- 从PIF制造工厂中分离出来的Cronobacter sakazakii菌株进行基因组特征.
- 在全球范围内调查这些菌株的毒性因素,抗生素耐药性和遗传相关性.
主要方法:
- 来自PIF植物的18种C. sakazakii分离物的全基因组测序 (2011-2015年).
- 遗传学分析,毒性基因鉴定和抗生素耐药性基因分析.
- 与96个公开的C. sakazakii基因组进行全球背景的比较.
主要成果:
- 序列类型1是分离物中占主导地位的类型.
- 所有的分离物体都具有毒性基因,并表现出对素和四环素的耐药性,具有强烈的基因型-表型相关性.
- 在分离物中确定了等离子体Col ((pHAD28) 和完整的菌体.
结论:
- 从PIF环境中对C. sakazakii的基因组洞察力揭示了疫情潜在的关键特征.
- 调查结果支持加强食品加工和公共卫生领域的监督和控制战略.
- 这项研究为了解C. sakazakii的流行病学和降低风险提供了基础.
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