对内菌 (Methylobacterium mesophilicum) 和病原体 (Xylella fastidiosa) 相互作用的转录组和秘密组分析表明营养素竞争
Manuella Nobrega Dourado1,2, Paulo Marques Pierry3, Oseias Rodrigues Feitosa-Junior3
1Microbiology Department, Biomedical Sciences Institute, University of Sao Paulo, Sao Paulo 05508-000, Brazil.
Microorganisms
|November 25, 2023
概括
甲基菌 (Methylobacterium mesophilicum) 通过争夺铁和等营养素并降解其细胞壁来抑制Xylella fastidiosa. 这种互动为管理类植物疾病和提高作物产量提供了策略.
科学领域:
- 植物病理学 植物病理学
- 微生物生态学 微生物生态学
- 分子生物学分子生物学
背景情况:
- 紫虫 (Xylella fastidiosa) 在水果和坚果作物中引起重大疾病.
- 甲基杆菌 (Methylobacterium mesophilicum SR1.6/6) 促进植物生长,并抑制了X. fastidiosa. 的生长.
- 这种微生物相互作用的分子机制在很大程度上是未知的.
研究的目的:
- 在共同培养中研究M. mesophilicum SR1.6/6和X. fastidiosa 9a5c之间的生理和分子相互作用.
- 阐明M. mesophilicum抑制X. fastidiosa的机制.
主要方法:
- 共同培养的微生物的转录组和分泌组分析.
- 鉴定差异表达的基因和分泌的蛋白质.
主要成果:
- X. fastidiosa高调压力和致病性基因 (例如,LesA) 和低调细胞分裂/运输基因.
- 梅索菲利克 (M. mesophilicum) 增加了铁的摄取,但降低了其他运输基因的摄取.
- M. mesophilicum分泌的蛋白质参与吸收和化酶,表明营养竞争和细胞壁退化.
结论:
- M. mesophilicum主要通过营养竞争 (铁,) 抑制X. fastidiosa,导致饥饿.
- 通过M. mesophilicum对X. fastidiosa细胞壁的酶降解有助于抑制.
- 了解这些相互作用为控制X. fastidiosa和提高果生产率提供了途径.
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