痕信号调节了Helicobacter pylori感染中的树突细胞的功能和表型
Qiaoyuan Liu1, Chuxi Chen1, Yunxuan He1
1School of Laboratory Medicine and Biotechnology, Southern Medical University, No. 1023, South Shatai Road, Baiyun District, Guangzhou 510515, China.
Microorganisms
|November 25, 2023
概括
在Helicobacter pylori感染期间,痕信号调节树突细胞 (DC) 功能. 阻断痕信号影响DC成熟和CD4+T细胞分化,促进Treg偏差反应.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 微生物学 微生物学
背景情况:
- 缺口信号对于树突细胞 (DC) 功能和T细胞相互作用至关重要.
- 诺奇信号在Helicobacter pylori (H. pylori) 感染中的作用尚不清楚.
研究的目的:
- 调查Notch信号在DC功能和T细胞分化在H. pylori感染期间的作用.
- 为了确定H. pylori如何影响DC中的Notch路径组件.
主要方法:
- 用H. pylori刺激小鼠骨髓衍生的树突细胞 (BMDCs),使用或不使用Notch抑制剂DAPT.
- 使用定量实时PCR (qRT-PCR),西斑,ELISA和流动细胞测量来测量Notch组分,细胞因子,表面标记物和T细胞分化.
主要成果:
- 在BMDCs中的H. pylori上调调节的Notch受体和配体 (Dll4,Jagged1).
- DAPT治疗影响了IL-1β和IL-10的产生,减少了Notch成分的表达,并降低了DC成熟标志物 (CD80,CD83,CD86,MHC-II).
- 缺口阻塞扭曲了CD4+T细胞对Treg细胞的分化.
结论:
- 在H. pylori感染期间,痕信号在调节DC功能和表型方面发挥着重要作用.
- 准痕信号影响DC成熟和CD4+T细胞分化,可能会影响对H. pylori的免疫反应.
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