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全基因组协会研究揭示了面包小麦中新型粉末菌抵抗位点

Ramandeep Kaur¹, Neeraj Kumar Vasistha^1,2, Vikas Kumar Ravat³

¹Department of Genetics-Plant Breeding and Biotechnology, Dr. Khem Sigh Gill Akal College of Agriculture, Eternal University, Baru Sahib, Sirmour 173101, India.

Plants (Basel, Switzerland)

|November 25, 2023

概括

这项研究确定了113个与面包小麦粉抗性相关的遗传标记. 染色体6B上的四个新型标记物显示出对这种常见的真菌病产生更有弹性的小麦品种的潜力.

科学领域:

植物病理学植物病理学
遗传学遗传学是一个
农业科学农业科学

背景情况:

粉状菌 (PM),是由 *Blumeria graminis* f. sp. 引起的 *tritici* (*Bgt*),对全球面包小麦产量构成重大威胁.
目前的小麦品种往往缺乏足够的对PM的抗性,因此需要确定新的抗性来源.
全基因组关联研究 (GWAS) 对于剖析疾病耐药性等复杂特征的遗传基础至关重要.

研究的目的:

进行全基因组关联研究 (GWAS),以确定与面包小麦粉耐药性相关的遗传位置.
发现新的标记特征关联 (MTA) 和候选对PM耐药性的基因.
为培育计划提供遗传资源,开发耐PM的小麦品种.

主要方法:

一个由286种面包小麦基因型组成的多样化小组在野外条件下连续三年评估了PM严重程度.
用Illumina 90K Infinium iSelect试验进行基因型识别,以生成全基因组单核酸多态性 (SNP) 数据.
使用FarmCPU多位置混合模型,以确定标志物-特征关联 (MTA) 的显著程度为*p* ≤0.001.

主要成果:

在染色体1A,1B,2B,3A,3B,4A,4B,5A,5B,6B,7A和7B上共发现了113种与PM耐药性相关的MTA.
在连续两年 (2020-2021年和2021-2022年) 连续检测到6B染色体上的四种新型MTA.
96个候选基因,包括编码蛋白激酶,富含白的重复和指蛋白的候选基因,在显著SNP的置信区间内被确定.

结论:

已识别的MTA和候选基因为增强面包小麦粉状真菌耐药性提供了宝贵的遗传资源.
染色体6B上的新型位点代表了在小麦育种计划中标记辅助选择的有希望的目标.
这项研究有助于开发可持续的策略,通过基因改进来管理小麦生产中的PM.

关键词:

农场的CPUCPU是什么在GWAS中,GWAS就是GWAS.更多关于MTAs 的文章候选基因是指候选基因的基因.粉状菌是一种粉状菌.小麦小麦小麦小麦小麦小麦小麦.

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Genome-wide Association Studies-GWAS

Genome-wide Association Studies-GWAS

Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...

Plant Breeding and Biotechnology

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