在中国出现的非典型猪瘟病毒的分离和分子表征
Hao Song1, Xiaowei Gao1, Yanhui Fu1
1State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 678 Haping Road, Harbin 150069, China.
Viruses
|November 25, 2023
概括
在中国的猪身上发现了一种新型病毒,即非典型的猪瘟病毒 (APPV),经常与猪病毒2型 (PCV2) 共感染. 一种新的APPV菌株被分离出来,有助于未来对这种新出现的猪病的研究和疫苗开发.
科学领域:
- 兽医病毒学 兽医病毒学
- 新兴的传染病 新兴的传染病
- 虫害病毒研究 虫害病毒研究
背景情况:
- 非典型的猪瘟病毒 (APPV) 是一种最近被发现的瘟病毒,在小猪中引起先天性震 (CT).
- 在Pestivirus属,Flaviviridae家族中,APPV具有高度分歧.
- 了解APPV的流行病学和特征对于猪健康至关重要.
研究的目的:
- 为了调查中国APPV的流行病学.
- 为了隔离和表征一种新的APPV菌株.
- 为进一步的APPV研究,诊断和疫苗开发提供资源.
主要方法:
- 来自中国的975个猪样本 (组织和血清) 的流行病学调查 (2017-2021年).
- RT-PCR,测序,免疫光测定和传输电子显微镜用于APPV隔离和确认.
- 细胞培养的传播和孤立的APPV菌株的全基因组测序.
主要成果:
- 在7.08%的样本中检测到APPV基因组.
- 观察到高的同时感染率,特别是猪肉病毒2型 (PCV2) (36.2%).
- 一种新型APPV菌株 (中国/HLJ491/2017) 已成功分离,测序,并以基因型2为特征.
结论:
- 这项研究为中国的APPV流行率和共感染模式提供了宝贵的见解.
- 单独分离纯净的APPV菌株为研究其病原性提供了关键资源.
- 这些发现支持开发新出现的APPV感染的诊断工具和疫苗.
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