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在活鸟中复合马雷克病病毒的表型表征验证了与病毒性相关的多态性

Taejoong Kim¹, Cari J Hearn², Jody Mays²

¹Southeast Poultry Research Laboratory, U.S. National Poultry Research Center, Agricultural Research Service, U.S. Department of Agriculture, 934 College Station Road, Athens, GA 30605, USA.

|November 25, 2023

概括

马雷克病病毒 (MDV) 的毒性与特定的遗传变异有关. 在MDV基因组中修改关键的单核酸多态 (SNP) 减少了疾病发病率,证实了它们在毒性中的作用.

科学领域:

兽医病毒学兽医病毒学
鸟类病理学鸟类病理学
分子遗传学分子遗传学

背景情况:

马雷克病 (MD) 是对家禽的重大经济威胁,由马雷克病病毒 (MDV) 引起.
MDV表现出不同的毒性,分为病型:轻度 (m),毒性 (v),非常毒性 (vv) 和非常毒性加 (vv+).
之前的研究已经确定了MDV基因组中的潜在的毒性相关单核酸多态 (SNP) 和基因.

研究的目的:

在八个开放式读取框架 (ORF) 中实验验验证特定非同义SNP (nsSNP) 对MDV毒性的贡献.
调查是否已识别的nsSNP足以改变MDV的毒性病理型.

主要方法:

通过用vMDV菌株中发现的核酸替换vv+MDV菌株686的基因组修饰.
在中进行致病性研究,以评估基因组修改对MD发病率和鸟类存活率的影响.
使用已确定的方法对改性MDV菌株的病毒性病态定型.

主要成果:

修改后的vv+MDV 686病毒在受挑战的鸟类中显示了减少的MD发病率和增加的生存率.
病变型分析表明,基因组置换将病毒性转移到类似vMDV的表型.
作为一个集体群体,测试的nsSNP不足以完全赋予vMDV病型.

结论:

在被调查的ORF中,非同义的SNP与马雷克病病毒毒性有关.
其他遗传变异可能有助于MDV复杂的毒性表型.
需要进一步的研究来确定影响MDV毒性的所有遗传因素.

关键词:

马雷克的疾病是马雷克的疾病.单核酸多形态的单核酸多形态病毒基因组病毒基因组毒性毒性是一种毒性.

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