通过量身定制的性真菌乳酸酶去聚化强力宁
David Rodríguez-Escribano1, Felipe de Salas1, Rocío Pliego1
1Centro de Investigaciones Biológicas Margarita Salas, Consejo Superior de Investigaciones Científicas (CSIC), 28040 Madrid, Spain.
Polymers
|November 25, 2023
概括
量身定制的 alkaliphilic laccases 可以在pH10下去聚合欧卡利普斯 kraft lignin. 一个膜分离系统防止了再聚合,使得有效的素分解可用于芳香回收.
科学领域:
- 生物技术是生物技术.
- 生物催化剂是一种生物催化剂.
- 绿色化学 绿色化学
背景情况:
- 纤维纤维厂的副产品 - - 实力纤维素是丰富的芳香化合物的来源.
- 菌乳酶适合于的脱聚合,但通常在酸性pH下发挥最佳作用.
- 需要性条件才能使强力素溶解,这对传统的酸构成了挑战.
研究的目的:
- 为了评估工程基酸乳酸酶对强电素脱聚合物的有效性.
- 研究这些酶在pH10时的氧化,脱甲基和脱聚合能力.
- 为了克服在单反应中的素重聚合问题.
主要方法:
- 酶导向的进化创造了高氧化还原潜力,性乳酸酶.
- 在pH 10下,用工程乳酸酶化白 kraft lignin.
- 功能组变化 (基,碳),甲醇释放和分子量分布的分析.
- 实施一个带有膜分离的基板级反应堆,以防止重聚合.
主要成果:
- 在pH10下,工程酸酶证明了 kraft素的氧化,脱甲基和脱聚合.
- 观察到基基和碳基基的增加,甲醇的释放,以及较低的分子量分数.
- 高度的酶和素导致重聚合,由增加的分子量和特定链接含量表明.
- 膜分离成功阻止了重聚合,证实了宁脱聚合.
结论:
- 工程制造的性乳酸酶是性条件下强力素脱聚合的有效生物催化剂.
- 通过集成分离防止重聚合对于高效的素分解至关重要.
- 这种方法增强了利用 kraft 素作为有价值的芳香化合物的来源的潜力.
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