在药物促进病例中检测和分析血中德克斯梅德托米丁
Wen-Ya Zhai1,2, Li-Na Wu1, Shuo Yang1
1Shanghai Key Laboratory of Forensic Medicine, Key Laboratory of Forensic Science, Ministry of Justice, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China.
Fa yi xue za zhi
|November 25, 2023
概括
一种新的,简单和快速的方法可以准确地检测血液中的德克斯梅德米丁. 这种经过验证的技术为临床和法医应用提供了可靠的定性和定量分析.
科学领域:
- 分析化学 分析化学
- 法医毒理学 法医毒理学
背景情况:
- 德克斯梅德托米丁是一种强大的镇静剂和止痛药.
- 在生物样本中精确检测对于临床监测和法医调查至关重要.
研究的目的:
- 开发一种简单的,快速的,定性和定量检测方法来检测血液中的德克斯梅德托米丁.
- 为了验证方法的性能特征.
主要方法:
- 血液样本通过酸沉和过进行处理.
- 使用PFP Propyl柱和同位素化液体色谱.
- 积极离子扫描模式和多反应监测 (MRM) 用于分析.
主要成果:
- 该方法实现了0.2 ng/mL的检测极限 (LOD) 和0.5 ng/mL的量化极限 (LOQ).
- 在0.5-1000 ng/mL范围内观察到出色的线性 (R2 > 0.99).
- 精度在90.34%至112.67%之间,提取回收率在50.05%至91.08%之间,没有显著的矩阵效应.
结论:
- 开发的方法简单,有选择性,适合在血液中对德克斯梅德托米丁进行分析.
- 这为涉及德克斯梅德托米丁的毒品犯罪调查提供了宝贵的工具.
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