阻塞性结直肠癌患者的细菌体和微生物群的变化:回顾性队列研究
Yinghao Cao1,2, Fumei Shang3, Min Jin2,4,5
1Department of Digestive Surgical Oncology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
BJS open
|November 25, 2023
概括
肠道阻塞的结肠直肠癌 (CRC) 与较差的患者存活率和独特的肠道微生物群概况有关. 具体来说,阻塞性CRC患者在瘤组织中显示出更高水平的Bacteroidetes.
科学领域:
- 胃肠病学 胃肠病学
- 在瘤学瘤学.
- 微生物学 微生物学
背景情况:
- 结直肠癌 (CRC),肠道阻塞和肠道微生物群之间的相互作用尚未得到充分理解.
- 研究肠道微生物群在阻塞性CRC中的作用对于了解疾病进展至关重要.
研究的目的:
- 在患有肠道阻塞的患者中,探索肠道微生物群变化和结直肠癌 (CRC) 之间的关联.
- 为了比较有或没有肠道阻塞的CRC患者的肠道微生物群组成.
主要方法:
- 倾向性得分匹配 (PSM) 用于比较313名有或没有阻塞的CRC患者.
- 16S rRNA测序分析了瘤和相邻的正常组织中的微生物组成.
- 在组织样本上进行了DNA提取和放大.
主要成果:
- 患有阻塞性CRC的患者的整体存活期和无病存活期 (DFS) 显著缩短.
- 阻塞CRC患者的瘤组织中,微生物的丰富性和多样性更高.
- 细菌体在受阻CRC患者的瘤组织中显著丰富,观察到明显的α和β多样性.
结论:
- 阻塞性结肠直肠癌 (CRC) 与更糟糕的预后和改变的肠道微生物群有关.
- 高水平的Bacteroides细菌是阻塞CRC的患者的一个关键特征.
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