疑似细菌感染中的Acinetobacter baumannii:多药耐药性,毒性基因和生物膜生成之间的关联
Deepan Gautam1, Karma Gurmey Dolma1, Bidita Khandelwal2
1Department of Microbiology, Sikkim Manipal University, Gangtok, Sikkim, India.
The Indian journal of medical research
|November 25, 2023
概括
博曼尼菌 (Acinetobacter baumannii) 是医院感染的常见原因,经常表现出多药性耐药性 (MDR) 和生物膜的产生. 这项研究发现MDR和临床隔离物中的生物膜形成之间存在显著的关联,突出显示了治疗方面的挑战.
科学领域:
- 临床微生物学 临床微生物学
- 传染性疾病 传染性疾病
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 宝曼尼菌 (Acinetobacter baumannii) 是一种重要的医院病原体,以高抗生素耐药性和生物膜形成而闻名.
- 了解毒性因子,抗生素耐药性和生物膜生产之间的相互作用对于管理这种细菌引起的感染至关重要.
研究的目的:
- 为了确定Acinetobacter baumannii在各种临床标本中的流行率.
- 研究生物膜生成,多药性耐药性 (MDR) 和A. baumannii.病毒性因子基因表达之间的关联.
主要方法:
- 通过检测类似于blaOXA-51的基因,确认了Acinetobacter baumannii的鉴定.
- 生物膜产量通过微位板测定进行评估.
- 病毒性基因,包括外膜蛋白A (OmpA) 和生物膜相关蛋白 (Bap),通过实时PCR量化.
主要成果:
- A. baumannii从307个临床标本中分离出来,主要来自内管 (44.95%).
- 观察到多重耐药性 (MDR) 的高率 (78.82%),其中66.78%的分离物产生生物膜.
- 在MDR和生物膜生产之间发现了显著的积极关联 (P<0.05),OmpA和Bap也与MDR积极相关.
结论:
- 来自下呼吸道标本的A. baumannii中MDR和生物膜形成的高患病率强调了其临床威胁.
- 碳烯抗性基因和生物膜生产的同时发生严重限制了治疗选择.
- 严格的抗微生物管理和分子监测对于打击医疗机构中耐药A. baumannii的传播至关重要.
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