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通过相结合的糖化-发酵素的生产聚基酸盐

Fernando Guzmán-Lagunes¹, Lorena Martínez-dlCruz², Phavit Wongsirichot³

¹Food Sciences and Biotechnology Department, Faculty of Chemistry, Universidad Nacional Autónoma de México, Mexico City, Mexico.

Bioprocess and biosystems engineering

|November 25, 2023

概括

草根的胰岛素可以通过水解和发酵产生多基酸盐 (PHB). 这项研究表明,胰岛素是PHB生产的可行的原料,产生比果糖更稳定的聚合物.

科学领域:

生物技术是生物技术.
聚合物科学聚合物科学
可再生资源可再生资源

背景情况:

胰岛素 (inulin) 是一种来自花等植物的果糖基多糖,是潜在的可再生果糖来源.
聚乙酸 (PHB) 是一种生物聚合物,具有多种应用.
与发酵相结合的胰岛素水解为PHB生产提供了一条途径.

研究的目的:

为了研究PHB从香草根内素的生产.
用Cupriavidus necator H16.16评估胰岛素水解和随后发酵的效率.
描述从胰岛素中产生的PHB,并将其与从纯果糖中产生的PHB进行比较.

主要方法:

奇科里根的胰岛素使用胰岛素酶Novozym 960进行了水解.
由此产生的水解酸被用作Cupriavidus necator H16发酵的基质.
分析了PHB产量,生物质度和聚合物特性.
研究了同时的糖化和发酵 (SSF).

主要成果:

在90分钟后,达到95%的最大胰岛素糖分化.
库普里亚维杜斯 (Cupriavidus necator) H16发酵产生了4g/L的生物质度,PHB积累率为30% (w/w).
有证据表明,同时进行了糖化和发酵过程.
与纯果糖PHB相比,从胰岛素生产的PHB具有更均的分子量和更好的热稳定性.

结论:

奇科里根的胰岛素是一种可行的替代碳基质,用于PHB生产.
该过程显示了利用富含胰岛素的可再生原料的潜力.
来自胰岛素的PHB显示了增强的特性,这表明它与基于果糖的生产相比具有优势.

关键词:

生物塑料是生物塑料.这就是Cupriavidus necator.酶性水解是一种酶性水解.在这种情况下,胰岛素和胰岛素是不同的,而胰岛素和胰岛素是不同的.聚基丁酸盐多基酸盐

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