Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

C4 Pathway and CAM01:27

C4 Pathway and CAM

45.6K
Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
45.6K
The Calvin Benson Cycle01:46

The Calvin Benson Cycle

4.6K
Ribulose 1,5- bisphosphate carboxylase/oxygenase (RuBisCo) is a critical enzyme that catalyzes carbon dioxide assimilation during photosynthesis. However, it is an inefficient enzyme, having an extremely slow catalytic rate. A typical enzyme can process about a thousand molecules per second; however, RuBisCo fixes only around three-carbon dioxides per second. Photosynthetic cells compensate for this slow rate by synthesizing very high amounts of RuBisCo, making it the most abundant single...
4.6K
The Calvin Cycle01:40

The Calvin Cycle

74.4K
Overview
74.4K
Overview of Nitrogen Metabolism01:20

Overview of Nitrogen Metabolism

8.0K
Nitrogen is a very important element for life because it is a major constituent of proteins and nucleic acids. It is a macronutrient, and in nature, it is recycled from organic compounds and stored in the form of  ammonia, ammonium ions, nitrate, nitrite, or  nitrogen gas by many metabolic processes. Many of these metabolic processes are carried out only by prokaryotes.
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
8.0K
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

214
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
214
¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

1.1K
The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
1.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Advancing biomarker development for chronic traumatic encephalopathy: Summary and recommendations from the 2025 Leon Thal Summit.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
Same author

Autoradiography and preclinical PET studies with radiolabeled asyn-44 and ACI-12589 for imaging α-synuclein.

Journal of Parkinson's disease·2026
Same author

25 Years of "Loop" Radiochemistry for PET Imaging.

Journal of labelled compounds & radiopharmaceuticals·2026
Same author

Tau-PET imaging and blood biomarkers reveal early tauopathy in special operations forces exposed to repetitive blast.

Brain communications·2026
Same author

Assessment of noninvasive quantification of [<sup>18</sup>F]SynVesT-1 with simplified reference tissue model 2 in an adolescent and youth cohort.

European journal of nuclear medicine and molecular imaging·2026
Same author

SV2A PET reveals synaptic density loss in experimental autoimmune encephalomyelitis and in a pilot multiple sclerosis study.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
查看所有相关文章

相关实验视频

Updated: Jul 10, 2025

Enhancing Efficiency and Radiolabeling Yields of Carbon-11 Radioligands for Clinical Research Using the Loop Method
09:08

Enhancing Efficiency and Radiolabeling Yields of Carbon-11 Radioligands for Clinical Research Using the Loop Method

Published on: December 20, 2024

1.4K

11C固定技术的使用方法

Emily Murrell1, Anton Lindberg1, Armando Garcia1

  • 1Azrieli Centre for Neuro-Radiochemistry, Brain Health Imaging Centre, Centre for Addiction and Mental Health, Toronto, ON, Canada.

Methods in molecular biology (Clifton, N.J.)
|November 25, 2023
PubMed
概括
此摘要是机器生成的。

这项研究介绍了一种用于放射性标记小分子的方法,使用循环仪产生的碳-11气体. 这些高效的碳-11固定技术使得各种放射性标记化合物的合成能够用于研究和生产.

关键词:
二氧化碳是二氧化碳中的一种.一氧化碳的一氧化碳.碳-11 碳-11 是一种碳.碳化是碳化的一种.炭基化是碳基化的一种.

更多相关视频

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
08:05

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Published on: October 7, 2020

6.2K
Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
07:26

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids

Published on: January 26, 2012

24.3K

相关实验视频

Last Updated: Jul 10, 2025

Enhancing Efficiency and Radiolabeling Yields of Carbon-11 Radioligands for Clinical Research Using the Loop Method
09:08

Enhancing Efficiency and Radiolabeling Yields of Carbon-11 Radioligands for Clinical Research Using the Loop Method

Published on: December 20, 2024

1.4K
Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
08:05

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Published on: October 7, 2020

6.2K
Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
07:26

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids

Published on: January 26, 2012

24.3K

科学领域:

  • 放射化学 放射化学是指辐射化学.
  • 核医学就是核医学.
  • 有机合成 有机合成

背景情况:

  • 碳-11 (11C) 是一个正子发射放射性核化物,对于正子发射断层扫描 (PET) 成像至关重要.
  • 为了开发新的PET放射性追踪剂,将C纳入目标分子的高效方法至关重要.

研究的目的:

  • 描述直接C-碳氧化和C-碳化反应的协议.
  • 介绍一种用于捕获循环子生成的[11C]CO2和[11C]CO.CO.的方法.

主要方法:

  • 使用循环生成的[C]CO2进行碳化和[C]CO进行碳化.
  • 采用在室温和大气压下捕获气体的单程序.
  • 为商用自动化合成单元调整协议.

主要成果:

  • 对许多小分子具有广泛的适用性.
  • 成功合成了各种C标记化合物,包括碳酸,胺,,,酸,酸,碳酸盐和尿素.
  • 建立了用于常规放射性药物生产的稳健方法.

结论:

  • 描述的C固定技术为放射性标记提供了一种多功能和高效的方法.
  • 该协议很容易适应C标记放射性药物的自动合成和常规生产.