解读由操作模式塑造的无氧乙醇代谢途径
Bang Du1, Xinmin Zhan1, Piet N L Lens2
1Civil Engineering, School of Engineering, College of Science and Engineering, University of Galway, Galway H91 TK33, Ireland.
Water research
|November 25, 2023
概括
测序批量反应器 (SBR) 增强了乙醇代谢和甲基生成. 长期添加粉状活性炭 (PAC) 会促进乙醇降解和酸生产,同时有利于在连续流反应堆 (CFR) 中进行 Geobacter 丰富.
科学领域:
- 微生物学 微生物学
- 环境科学 环境科学
- 生物技术是生物技术.
背景情况:
- 有效的无氧消化依赖于微生物同和多样化的代谢途径.
- 了解乙醇代谢途径对于优化生物气生产至关重要.
研究的目的:
- 为了研究测序批量反应堆 (SBR) 和连续流动反应堆 (CFR) 运行模式,有或没有粉末活性炭 (PAC) 对乙醇代谢途径的影响.
- 确定调节无氧乙醇代谢和丰富特定微生物群落的策略,如Geobacter.
主要方法:
- 用乙醇养的SBR和CFR系统的比较.
- 粉末活性炭 (PAC) 添加效应的评估 (短期和长期).
- 分析微生物群落的组成,酶活性 (EC: 1.2.7.1,EC: 1.2.7.11),污泥导电性和代谢速率 (乙醇降解,甲基生成,酸生产/降解).
主要成果:
- SBR的运行,特别是与CO2的运行,有利于乙醇代谢转向酸盐,由Desulfobulbus的主导地位和增加CO2代谢酶证明.
- 与CFR相比,SBR显示以生物质为基础的乙醇降解率 (53.1%) 和甲基生成率 (22.3%) 显著更高.
- 在CFR中延长固体保留时间丰富了Geobacter (71.7%和70.4%与/或没有PAC).
- 长期的PAC添加增强了乙醇降解和酸盐代谢,而短期和长期的添加减少了甲生成滞后阶段和增加了污泥导电性.
结论:
- 操作模式 (SBR与CFR) 和PAC添加是控制无氧乙醇代谢途径的有效策略.
- SBRs促进了酸盐的产生,并提高了整体降解和甲基生成的速度.
- 长期的PAC添加有利于改善乙醇降解和酸盐代谢,并且具有延长固体保留时间的CFR可以为Geobacter丰富.
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